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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genetic background affects the expansion of macrophage subsets in the lungs of Mycobacterium tuberculosis-infected hosts

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Autor(es):
Bertolini, Thais Barboza [1] ; de Souza, Alexandre Ignacio [1] ; Gembre, Ana Flavia [1] ; Pineros, Annie Rocio [1] ; Prado, Rafael de Queiroz [1] ; Silva, Joao Santana [1] ; Zambelli Ramalho, Leandra Naira [2] ; Deperon Bonato, Vania Luiza [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Immunology; v. 148, n. 1, p. 102-113, MAY 2016.
Citações Web of Science: 7
Resumo

M1 macrophages are more effective in the induction of the inflammatory response and clearance of Mycobacterium tuberculosis than M2 macrophages. Infected C57BL/6 mice generate a stronger cellular immune response compared with BALB/c mice. We hypothesized that infected C57BL/6 mice would exhibit a higher frequency and function of M1 macrophages than infected BALB/c mice. Our findings show a higher ratio of macrophages to M2 macrophages in the lungs of chronically infected C57BL/6 mice compared with BALB/c mice. However, there was no difference in the functional ability of M1 and M2 macrophages for the two strains in vitro. In vivo, a deleterious role for M2 macrophages was confirmed by M2 cell transfer, which rendered the infected C57BL/6, but not the BALB/c mice, more susceptible and resulted in mild lung inflammation compared with C57BL/6 mice that did not undergo cell transfer. M1 cell transfer induced a higher inflammatory response, although not protective, in infected BALB/c mice compared with their counterparts that did not undergo cell transfer. These findings demonstrate that an inflammation mediated by M1 macrophages may not induce bacterial tolerance because protection depends on the host genetic background, which drives the magnitude of the inflammatory response against M. tuberculosis in the pulmonary microenvironment. The contribution of our findings is that although M1 macrophage is an effector leucocyte with microbicidal machinery, its dominant role depends on the balance of M1 and M2 subsets, which is driven by the host genetic background. (AU)

Processo FAPESP: 12/14524-9 - Modulação da diferenciação de linfócitos T em infecções por protozoários, fungos e bactérias
Beneficiário:João Santana da Silva
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/09702-2 - Avaliação do papel das células dendríticas na diferenciação de populações de células CD4+ em animais com capacidade diferente de elaborar resposta imune celular contra Mycobacterium tuberculosis
Beneficiário:Vânia Luiza Deperon Bonato
Linha de fomento: Auxílio à Pesquisa - Regular