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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants

Texto completo
Autor(es):
Vicente, Filipa A. [1] ; Lario, Luciana D. [2] ; Pessoa, Jr., Adalberto [2] ; Ventura, Sonia P. M. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro - Portugal
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Process Biochemistry; v. 51, n. 4, p. 528-534, APR 2016.
Citações Web of Science: 11
Resumo

Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 12/23726-4 - "purificação de enzimas proteolíticas secretadas pela levedura psicrótrofa Rhodoturola mucilaginosa por sistemas de duas fases aquosas (PEG/NaPA)e processos cromatográficos"
Beneficiário:Luciana Daniela Lario
Linha de fomento: Bolsas no Brasil - Pós-Doutorado