| Texto completo | |
| Autor(es): |
Torres, Lidiane de Souza
[1]
;
Okumura, Jessika Viviani
[1]
;
Humberto da Silva, Danilo Grunig
[1]
;
Belini Junior, Edis
[1]
;
de Oliveira, Renan Garcia
[1]
;
Oliveira Mimura, Kallyne Kioko
[2]
;
de Castro Lobo, Clarisse Lopes
[3]
;
Oliani, Sonia Maria
[2]
;
Bonini Domingos, Claudia Regina
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ Unesp, Dept Biol, Lab Hemoglobin & Hematol Genet Dis, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Sao Paulo State Univ Unesp, Dept Biol, Lab Imunomorphol, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] Inst Hematol Arthur Siqueira Cavalcanti Hemorio, Rua Frei Caneca 08, BR-20211030 Rio De Janeiro, RJ - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CYTOKINE; v. 80, p. 18-25, APR 2016. |
| Citações Web of Science: | 4 |
| Resumo | |
Sickle cell disease (SCD) represents a chronic inflammatory condition with complications triggered by the polymerization of hemoglobin S (Hb S), resulting in a series of cellular interactions mediated by inflammatory cytokines, as the transforming growth factor beta (TGF-beta), which plays an important role in inflammation resolution. This study assessed the relation between SCD inflammation and the plasma concentration of TGF-beta 1, and also checked the influence of the presence of 509C/T polymorphism in TGFB1 gene on TGF-beta 1 plasma values. The plasma levels of TGF-beta 1 were quantified by ELISA in 115 patients with SCD (genotypes SS, SD-Los Angeles, S beta-thalassemia and SC) and in 58 individuals with no hemoglobinopathies (Hb AA), as the control group. The 509C/T polymorphism in TGFB1 gene was screened by PCR-RFLP. The correlation between TGF-beta 1 plasma levels and the inflammation was based on its association with the count of platelets, total white blood cells (WBC) and neutrophils in the peripheral blood. Patients with SCD showed plasma levels of TGF-beta 1 higher than the control group, especially the Hb SS genotype, followed by the group with Hb SD. Polymorphism investigation showed no interference in the values obtained for the cytokine in the groups evaluated. All SCD groups showed TGF-beta 1 levels positively correlated to the platelets and WBC counts. The original data obtained in this study for SCD support the involvement of TGF-beta 1 in regulating of the inflammatory response and suggest that this marker possibly may become a potential therapeutic target in the treatment of the disease. (C) 2016 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 12/19653-1 - Polimorfismos genéticos e expressão de marcadores envolvidos em processos inflamatórios, angiogênicos e de hipóxia na doença falciforme |
| Beneficiário: | Lidiane de Souza Torres |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |