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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Differential DNA Methylation of MicroRNA Genes in Temporal Cortex from Alzheimer's Disease Individuals

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Autor(es):
Villela, Darine [1] ; Ramalho, Rodrigo F. [2] ; Silva, Aderbal R. T. [2] ; Brentani, Helena [3] ; Suemoto, Claudia K. [4, 5] ; Pasqualucci, Carlos Augusto [4, 6] ; Grinberg, Lea T. [4, 7] ; Krepischi, Ana C. V. [1] ; Rosenberg, Carla [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Inst Biosci, Rua Matao 277, BR-05508090 Sao Paulo, SP - Brazil
[2] AC Camargo Hosp, Int Res Ctr, CIPE, Rua Tagua 440, BR-01508010 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Inst & Dept Psychiat, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Sch Med, Brazilian Aging Brain Study Grp, LIM22, Dept Pathol, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Sch Med, Discipline Geriatr, Dept Internal Med, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[6] Univ Sao Paulo, Sch Med, Dept Pathol, Ave Doutor Arnaldo 455, BR-01246000 Sao Paulo, SP - Brazil
[7] Univ Calif San Francisco, Memory & Aging Ctr, Dept Neurol, 675 Nelson Rising Lane, POB 1207, San Francisco, CA 94143 - USA
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: NEURAL PLASTICITY; 2016.
Citações Web of Science: 12
Resumo

This study investigated for the first time the genomewide DNA methylation changes of noncoding RNA genes in the temporal cortex samples from individuals with Alzheimer's disease (AD). The methylome of 10 AD individuals and 10 age-matched controls were obtained using Illumina 450K methylation array. A total of 2,095 among the 15,258 interrogated noncoding RNA CpG sites presented differential methylation, 161 of which were associated with miRNA genes. In particular, 10 miRNA CpG sites that were found to be hypermethylated in AD compared to control brains represent transcripts that have been previously associated with the disease. This miRNA set is predicted to target 33 coding genes from the neuregulin receptor complex (ErbB) signaling pathway, which is required for the neurons myelination process. For 6 of these miRNA genes (MIR9-1, MIR9-3, MIR181C, MIR124-1, MIR146B, and MIR451), the hypermethylation pattern is in agreement with previous results from literature that shows downregulation of miR-9, miR-181c, miR-124, miR-146b, and miR-451 in the AD brain. Our data implicate dysregulation of miRNA methylation as contributor to the pathogenesis of AD. (AU)

Processo FAPESP: 09/00898-1 - Desequilíbrios genômicos submicroscópicos em quadros clínicos específicos de anomalias congênitas e deficiência mental
Beneficiário:Carla Rosenberg
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 10/15503-0 - Desequilíbrios genômicos nas manifestações anatomopatológicas e cognitivas da Doença de Alzheimer
Beneficiário:Darine Christina Maia Villela
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/08028-1 - CEGH-CEL - Centro de Estudos do Genoma Humano e de Células-Tronco
Beneficiário:Mayana Zatz
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs