Thyroid hormone reduces inflammatory cytokines improving glycaemia control in alloxan-induced diabetic wistar rats

Autor(es)

Panveloski-Costa, A. C. ^[1] ; Teixeira, S. Silva ^[1] ; Ribeiro, I. M. R. ^[1] ; Serrano-Nascimento, C. ^[1] ; das Neves, R. X. ^[2] ; Favaro, R. R. ^[2] ; Seelaender, M. ^[2] ; Antunes, V. R. ^[1] ; Nunes, M. T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA PHYSIOLOGICA; v. 217, n. 2, p. 130-140, JUN 2016.
• Citações Web of Science: 12

Resumo

Aim: This study aimed at evaluating whether thyroid hormone treatment could improve glycaemia and insulin response in alloxan-induced diabetic rats by altering cytokine expression in the skeletal muscle and epididymal white adipose tissue (eWAT) as well as altering inflammatory cell infiltration in eWAT. Methods: Diabetes mellitus (DM) was induced in male Wistar rats by alloxan injection, and a subset of the diabetic rats was treated with T3 (1.5 mu g per 100 g body weight) for a 28-day period (DT3). Cytokines were measured in serum (MILIplex assay kit) as well as in soleus and EDL skeletal muscles and eWAT by Western blotting. Thyroid function was evaluated by morphological, molecular and biochemical parameters. Cardiac function was assessed by measuring heart rate, blood pressure, maximal rate of pressure development (dp/dt(max)) and decline (dp/dt(min)) as well as the contractility index (CI). Sixty rats were used in the study. Results: Diabetic rats exhibited decreased thyroid function and increased inflammatory cytokines in serum, soleus muscle and eWAT. T3 treatment decreased glycaemia and improved insulin sensitivity in diabetic animals. These alterations were accompanied by decreased TNF-alpha and IL-6 content in soleus muscle and eWAT, and inflammatory cell infiltration in eWAT. T3 treatment did not affect cardiac function of diabetic rats. Conclusions: The present data provide evidence that T3 treatment reduces glycaemia and improves insulin sensitivity in diabetic rats, and that at least part of this effect could result from its negative modulation of inflammatory cytokine expression. (AU)

• Processo FAPESP: 10/18151-7 - Caracterização das ações dos HT e do seu agonista GC-1 na regulação da homeostase glicêmica: estudo em células musculares e em ratos diabéticos
• Beneficiário: Silvania da Silva Teixeira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/09014-9 - Efeitos do hormônio tireoidiano na modulação da expressão de citocinas envolvidas na sinalização da insulina na vigência de obesidade ou diabetes
• Beneficiário: Ana Carolina Panveloski Costa Salomão
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/05629-4 - Ações genômicas vs não genômicas dos hormônios tiroidianos: mudanças de paradigmas, implicações fisiológicas e perspectivas terapêuticas
• Beneficiário: Maria Tereza Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Temático