Unique behavior of Trypanosoma cruzi mevalonate kinase: A conserved glycosomal enzyme involved in host cell invasion and signaling

Autor(es):Mostrar menos -

Ferreira, Eden Ramalho ^[1] ; Horjales, Eduardo ^[2] ; Bonfim-Melo, Alexis ^[1] ; Cortez, Cristian ^[1] ; da Silva, Claudio Vieira ^[3] ; De Groote, Michel ^[2] ; Paschoal Sobreira, Tiago Jose ^[4] ; Cruz, Mario Costa ^[1] ; Lima, Fabio Mitsuo ^[1] ; Cordero, Esteban Mauricio ^[1] ; Yoshida, Nobuko ^[1] ; da Silveira, Jose Franco ^[1] ; Mortara, Renato Arruda ^[1] ; Bahia, Diana ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis, Sao Carlos, SP - Brazil

^[3] Univ Fed Uberlandia, Inst Ciencias Biomed, Av Engenheiro Dinz 1178, CP 593, BR-38400 Uberlandia, MG - Brazil

^[4] Lab Nacl Biociencias, Campinas, SP - Brazil

^[5] Univ Fed Minas Gerais, Inst Ciencias Biol, Dep Biol Geral, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, APR 26 2016.
• Citações Web of Science: 9

Resumo

Mevalonate kinase (MVK) is an essential enzyme acting in early steps of sterol isoprenoids biosynthesis, such as cholesterol in humans or ergosterol in trypanosomatids. MVK is conserved from bacteria to mammals, and localizes to glycosomes in trypanosomatids. During the course of T. cruzi MVK characterization, we found that, in addition to glycosomes, this enzyme may be secreted and modulate cell invasion. To evaluate the role of TcMVK in parasite-host cell interactions, TcMVK recombinant protein was produced and anti-TcMVK antibodies were raised in mice. TcMVK protein was detected in the supernatant of cultures of metacyclic trypomastigotes (MTs) and extracellular amastigotes (EAs) by Western blot analysis, confirming its secretion into extracellular medium. Recombinant TcMVK bound in a non-saturable dose-dependent manner to HeLa cells and positively modulated internalization of T. cruzi EAs but inhibited invasion by MTs. In HeLa cells, TcMVK induced phosphorylation of MAPK pathway components and proteins related to actin cytoskeleton modifications. We hypothesized that TcMVK is a bifunctional enzyme that in addition to playing a classical role in isoprenoid synthesis in glycosomes, it is secreted and may modulate host cell signaling required for T. cruzi invasion. (AU)

• Processo FAPESP: 11/51475-3 - Biologia molecular e celular do parasitismo por Trypanosoma cruzi
• Beneficiário: José Franco da Silveira Filho
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/50551-2 - Identificação e caracterização molecular de proteínas quinases de Trypanosoma cruzi para o estudo da comunicação celular, modelagem molecular e desenho de drogas inibidoras: estudo dos parceiros das vias de sinalização focado na invasão de EA
• Beneficiário: Diana Bahia
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores