Comparative analysis of expression profiling of the trypsin and chymotrypsin genes from Lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors

Autor(es)

Souza, Thais P. ^[1] ; Dias, Renata O. ^[1] ; Castelhano, Elaine C. ^[1] ; Brandao, Marcelo M. ^[2] ; Moura, Daniel S. ^[3] ; Silva-Filho, Marcio C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11, BR-13400918 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Ave Candido Rondon 400, BR-13083875 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Ave Padua Dias 11, BR-13400918 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 196, p. 67-73, JUN-JUL 2016.
• Citações Web of Science: 4

Resumo

Peptidase inhibitors (Pis) are essential proteins involved in plant resistance to herbivorous insects, yet many insect species are able to escape the negative effects of these molecules. We compared the effects of acute and chronic ingestion of soybean peptidase inhibitors (SPIs) on Spodoptera frugiperda and Diatraea saccharalis, two Lepidoptera species with different sensitivities to SPI ingestion. We analyzed the trypsin and chymotrypsin gene expression profiles in both species. Acute exposure of S. frugiperda to the inhibitors activated seven genes (SfChy5, SfChy9, SfChy19, SfChy22, SfTry6, SfTry8, and SfTry10), whereas chronic exposure activated 16 genes (SfChy2, SfChy4, SfChy5, SfChy8, SfChy9, SfChy11, SfChy12, SfChy15, SfChy17, SfChy21, SfChy22, SfTry6, SfTry8, SfTry9, SfTry10, and SfTry12). By contrast, the challenge of D. saccharalis with SPIs did not differentially induce the expression of trypsin- or chymotrypsin-encoding genes, with the exception of DsChy7. Bayesian phylogenetic analysis of S. frugiperda trypsin protein sequences revealed two gene clades: one composed of genes responsive to the SPIs and a second composed of the unresponsive genes. D. saccharalis trypsin proteins were clustered nearest to the S. frugiperda unresponsive genes. Overall, our findings support a hypothesized mechanism of resistance of Noctuidae moths to SPIs, involving gene number expansion of trypsin and chymotrypsin families and regulation of gene expression, which could also explain the variable susceptibility between S. frugiperda and D. saccharalis to these plant inhibitors. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/00417-3 - Biologia de sistemas aplicada a agricultura: análise de transcriptomas e interactomas
• Beneficiário: Marcelo Mendes Brandao
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/03040-0 - Evolução e caracterização da família gênica das serina proteinases e sua associação ao comportamento alimentar em insetos da família Noctuidae
• Beneficiário: Renata de Oliveira Dias
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 08/52067-3 - Herbivoria e o transporte intracelular de proteínas
• Beneficiário: Márcio de Castro Silva Filho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 09/15421-6 - Decifrando os mecanismos adaptativos de Spodoptera frugiperda aos inibidores de proteinases de plantas
• Beneficiário: Thais Paula de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/17110-5 - Caracterização das bases moleculares envolvidas no polifagismo de insetos herbívoros
• Beneficiário: Renata de Oliveira Dias
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático