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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The effect of white light on normal and malignant murine melanocytes: A link between opsins, clock genes, and melanogenesis

Texto completo
Autor(es):
de Assis, L. V. M. [1] ; Moraes, M. N. [1] ; da Silveira Cruz-Machado, S. [2] ; Castrucci, A. M. L. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Physiol, Inst Biosci, Lab Comparat Physiol Pigmentat, R Matao, Trav 14, 101, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Physiol, Inst Biosci, Lab Chronopharmacol, R Matao, Trav 14, 101, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH; v. 1863, n. 6, A, p. 1119-1133, JUN 2016.
Citações Web of Science: 19
Resumo

The skin possesses a photosensitive system comprised of opsins whose function is not fully understood, and clock genes which exert an important regulatory role in skin biology. Here, we evaluated the presence of opsins in normal (Melan-a cells) and malignant (B16-F10 cells) murine melanocytes. Both cell lines express Opn2, Opn4 - for the first time reported in these cell types - as well as S-opsin. OPN4 protein was found in a small area capping the cell nuclei of B16-F10 cells kept in constant dark (DD); twenty-four hours after the white light pulse (WLP), OPN4 was found in the cell membrane. Despite the fact that B16-F10 cells expressed less Opn2 and Opn4 than Melan-a cells, our data indicate that the malignant melanocytes exhibited increased photoresponsiveness. The clock gene machinery is also severely downregulated in B16-F10 cells as compared to Melan-a cells. Pert, Per2, and Bmal1 expression increased in B16-F10 cells in response to WLP. Although no response in clock gene expression to WLP was observed in Melan-a cells, gene correlational data suggest a minor effect of WLP. In contrast to opsins and clock genes, melanogenesis is significantly upregulated in malignant melanocytes in comparison to Melan-a cells. Tyrosinase expression increased after WLP only in B16-F10 cells; however no increase in melanin content after WLP was seen in either cell line. Our findings may prove useful in the treatment and the development of new pharmacological approaches of depigmentation diseases and skin cancer. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/24337-4 - Mecanismos de modulação de genes de relógio em melanócitos normais (melan-A) e transformados (melanoma B16-F10) por UVA e luz azul
Beneficiário:Leonardo Vinícius Monteiro de Assis
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 14/16412-9 - Mecanismos de termo-modulação de genes de relógio em tecidos periféricos de mamíferos: papel dos canais TRP
Beneficiário:Maria Nathália de Carvalho Magalhães Moraes Figueira Borges
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/50214-4 - Mecanismos de ajuste do relógio por luz e temperatura: aspectos filogenéticos
Beneficiário:Ana Maria de Lauro Castrucci
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/04557-5 - A ativação do eixo imune-pineal por dieta rica em lipídeos
Beneficiário:Sanseray da Silveira Cruz Machado
Linha de fomento: Bolsas no Brasil - Pós-Doutorado