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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Low-intensity laser phototherapy enhances the proliferation of dental pulp stem cells under nutritional deficiency

Texto completo
Autor(es):
Moura-Netto, Cacio [1] ; Ferreira, Leila Soares [2] ; Maranduba, Carlos Magno [3] ; Volpi Mello-Moura, Anna Carolina [2] ; Marques, Marcia Martins [4]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Cruzeiro Sul, Sch Dent, Sao Paulo, SP - Brazil
[2] Univ Ibirapuera, Sch Dent, Sao Paulo, SP - Brazil
[3] Univ Fed Juiz de Fora, Inst Biol Sci, Dept Biol, Juiz De Fora, MG - Brazil
[4] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Brazilian Oral Research; v. 30, n. 1 2016.
Citações Web of Science: 7
Resumo

Dental trauma in immature permanent teeth can damage pulp vascularization, which leads to necrosis and cessation of apexogenesis. Studies on tissue engineering using stem cells from human exfoliated deciduous teeth (SHEDs) have yielded promising results. Laser phototherapy (LPT) is able to influence the proliferation and differentiation of these cells, which could improve tissue engineering. SHEDs (eighth passage) were seeded into 96-well culture plates (103 cells/well) and were grown in culture medium supplemented with 15% defined fetal bovine serum (FBS) for 12 h. After determining the appropriate nutrition deficiency status (5% FBS), the cells were assigned into four groups: 1) G1 - 15% FBS (positive control); 2) G2 - 5% FBS (negative control); 3) G3 - 5% FBS+LPT 3 J/cm(2); and 4) G4 - 5% FBS+LPT 5 J/cm(2). For the LPT groups, two laser irradiations at 6 h intervals were performed using a continuous wave InGaAlP diode laser (660 nm, with a spot size of 0.028 cm(2), 10 mW) in punctual and contact mode. Cell viability was assessed via an MTT reduction assay immediately after the second laser irradiation (0 h) and 24, 48, and 72 h later. We found that G3 and G4 presented a significantly higher cell growth rate when compared with G2 (p < 0.01). Moreover, G4 exhibited a similar cell growth rate as G1 throughout the entire experiment (p > 0.05). These findings indicate that LPT with 5 J/cm(2) can enhance the growth of SHEDs during situations of nutritional deficiency. Therefore, LPT could be a valuable adjunct treatment in tissue engineering when using stem cells derived from the dental pulp of primary teeth. (AU)

Processo FAPESP: 10/12458-3 - Análise do efeito da fototerapia com laser em baixa intensidade em células-tronco de polpa dentária humana de dente decíduo. Parte 1: determinação das curvas de crescimento celular em função da suplementação nutricional do meio de cultivo
Beneficiário:Francielli Quintela dos Santos
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 09/13483-4 - Análise do efeito da fototerapia com laser em baixa intensidade em células-tronco de polpa dentária humana de dente decíduo (SHED-LacZ): Estudo in vitro e in vivo da produção de proteínas da matriz dentinária
Beneficiário:Cacio de Moura Netto
Linha de fomento: Bolsas no Brasil - Pós-Doutorado