Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Reduced insulin secretion function is associated with pancreatic islet redistribution of cell adhesion molecules (CAMs) in diabetic mice after prolonged high-fat diet

Texto completo
Autor(es):
Falcao, Viviane Tannuri F. L. [1, 2] ; Maschio, Daniela A. [1] ; de Fontes, Camila Calvo [3] ; Oliveira, Ricardo B. [1] ; Santos-Silva, Junia C. [4, 1] ; Soares Almeida, Anna Carolina [5] ; Vanzela, Emerielle C. [4] ; Cartaxo, Maria Tereza [2] ; Carvalho, Carolina P. F. [3] ; Collares-Buzato, Carla Beatriz [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Pernambuco UPE, Coll Nursing, Inst Biol, Recife, PE - Brazil
[3] Univ Fed Sao Paulo, Dept Biosci, Santos, SP - Brazil
[4] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP - Brazil
[5] Fed Univ Pernambuco UFRPE, Dept Biol, Recife, PE - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Histochemistry and Cell Biology; v. 146, n. 1, p. 13-31, JUL 2016.
Citações Web of Science: 13
Resumo

Intercellular junctions play a role in regulating islet cytoarchitecture, insulin biosynthesis and secretion. In this study, we investigated the animal metabolic state as well as islet histology and cellular distribution/expression of CAMs and F-actin in the endocrine pancreas of C57BL/6/JUnib mice fed a high-fat diet (HFd) for a prolonged time period (8 months). Mice fed a HFd became obese and type 2 diabetic, displaying significant peripheral insulin resistance, hyperglycemia and moderate hyperinsulinemia. Isolated islets of HFd-fed mice displayed a significant impairment of glucose-induced insulin secretion associated with a diminished frequency of intracellular calcium oscillations compared with control islets. No marked change in islet morphology and cytoarchitecture was observed; however, HFd-fed mice showed higher beta cell relative area in comparison with controls. As shown by immunohistochemistry, ZO-1, E-, N-cadherins, alpha- and beta-catenins were expressed at the intercellular contact site of endocrine cells, while VE-cadherin, as well as ZO-1, was found at islet vascular compartment. Redistribution of N-, E-cadherins and alpha-catenin (from the contact region to the cytoplasm in endocrine cells) associated with increased submembranous F-actin cell level as well as increased VE-cadherin islet immunolabeling was observed in diabetic mice. Increased gene expression of VE-cadherin and ZO-1, but no change for the other proteins, was observed in islets of diabetic mice. Only in the case of VE-cadherin, a significant increase in islet content of this CAM was detected by immunoblotting in diabetic mice. In conclusion, CAMs are expressed by endocrine and endothelial cells of pancreatic islets. The distribution/expression of N-, E- and VE-cadherins as well as alpha-catenin and F-actin is significantly altered in islet cells of obese and diabetic mice. (AU)

Processo FAPESP: 00/05137-4 - Avaliação da expressão localização e função de proteínas sinalizadoras com microscopia confocal
Beneficiário:Sara Teresinha Olalla Saad
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 10/50789-1 - Papel do contato célula-célula mediado pelas junções intercelulares no processo de maturação e disfunção das células beta do pâncreas
Beneficiário:Carla Beatriz Collares Buzato
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/54129-9 - EMU: Laboratório Central de Tecnologias de Alto Desempenho em ciências da vida
Beneficiário:Paulo Arruda
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários