Hyaluronidase decreases neutrophils infiltration to the inflammatory site

Autor(es)

Fronza, Marcio ^{[1, 2]} ; Muhr, Cornelia ^[3] ; Calheiros da Silveira, Denise Sayuri ^[1] ; Sorgi, Carlos Arterio ^[1] ; de Paula Rodrigues, Stephen Fernandes ^[4] ; Poliselli Farsky, Sandra Helena ^[4] ; Garcia Paula-Silva, Francisco Wanderley ^[1] ; Merfort, Irmgard ^[3] ; Faccioli, Lucia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Vila Velha, Dept Farm, Vila Velha, Espirito Santo - Brazil

^[3] Univ Freiburg, Dept Pharmaceut Biol & Biotechnol, Hugstetter Str 55, D-79106 Freiburg - Germany

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Inflammation Research; v. 65, n. 7, p. 533-542, JUL 2016.
• Citações Web of Science: 6

Resumo

Objective To evaluate the in vivo anti-inflammatory potential of bovine hyaluronidase (HYAL) using two different models of acute inflammation. Methods Air pouches were produced in the dorsal subcutaneous of mice and injected with phosphate saline solution or HYAL. The antiinflammatory action of HYAL was evaluated in carrageenan (Cg)-inflamed air pouches. After 4 and 24 h the cellular influx, protein exudation, cytokines and lipid mediators were evaluated. The action of HYAL on the rolling and adhesion of leukocytes was investigated in the LPS-stimulated mesenteric microcirculation by intravital microscopic. Results Treatment with HYAL reduced the cellular influx and protein exudation in non-inflamed and inflamed air pouches. HYAL treatment of Cg-inflamed air pouch reduced the production of tumor necrosis factor-alpha (TNF-alpha), interleukin-8 (IL-8), leukotriene B4 (LTB4) and LTC4, whereas prostaglandins E-2 (PGE(2)) and D-2 (PGD(2)) concentrations were unchanged. Histological analyses showed that HYAL administration diminished cell infiltration in the air-pouch lining. In LPS-stimulated mesenteric microcirculation, HYAL usage decreased rolling and adhesion of leukocytes, but did not affect the blood vessels diameters. Conclusion The results demonstrate that HYAL inhibited cellular recruitment, edema formation and pro-inflammatory mediators production, resulting in decreased adherence of leukocytes to blood vessels and tissue infiltration. Our data suggest that HYAL may be considered an effective candidate to ameliorate acute inflammation. (AU)

• Processo FAPESP: 09/07169-5 - Mediadores lipídicos como reguladores da resposta imune
• Beneficiário: Lúcia Helena Faccioli
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/23992-3 - Avaliação da atividade biológica da enzima hialuronidase e sua possível participação no processo de cicatrização cutânea
• Beneficiário: Marcio Fronza
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado