Influence of Restoration Type on the Cytotoxicity of a 35% Hydrogen Peroxide Bleaching Gel

Autor(es)

Soares, D. G. ^[1] ; Marcomini, N. ^[1] ; Basso, F. G. ^[1] ; Pansani, T. N. ^[2] ; Hebling, J. ^[3] ; de Souza Costa, C. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Physiol & Pathol, Araraquara Sch Dent, Sao Paulo - Brazil

^[2] Univ Estadual Paulista, UNESP, Dept Dent Mat & Prosthodont, Araraquara Sch Dent, Sao Paulo - Brazil

^[3] Univ Estadual Paulista, UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Operative Dentistry; v. 41, n. 3, p. 293-304, MAY-JUN 2016.
• Citações Web of Science: 0

Resumo

Objectives: The tooth/restoration interface may act as a pathway for hydrogen peroxide (H2O2) diffusion into the pulp chamber. Therefore, the influence of resin-modified glass ionomer cement (RMGIC) and resin composite simulated restorations on the cytotoxicity of an in-office bleaching gel was assessed in vitro. Materials and Methods: Cavities in enamel/dentin discs restored with RMGIC Vitremer (3M ESPE) or Single Bond/Filtek Z350 (3M ESPE) resin composite (RC) were subjected or not subjected to hydrolytic degradation (HD). A 35%-H2O2 bleaching gel was applied to simulated restored and nonrestored enamel surfaces, and culture medium in contact with the dentin substrate (extract) was collected and applied to MDPC-23 cells. Nonrestored discs subjected or not subjected to bleaching were used as positive and negative controls, respectively. Cell viability, oxidative stress, interleukin (IL)-1b expression, alkaline phosphatase (ALP) activity, and mineralized nodule deposition were evaluated. The H2O2 in the extracts was quantified. Data were subjected to statistical analysis. Results: Higher oxidative stress associated with reduced cell viability, ALP activity, and mineralized nodule deposition was observed for all bleached groups compared with the negative control group. The RMGIC/HD group, which presented the highest H2O2 diffusion, had the lowest values of cell viability, ALP activity, and mineralized nodule deposition, as well as significantly increased IL-1 beta expression. Conclusions: Dental cavities restored with the RMGIC subjected to hydrolytic degradation allowed for more intense diffusion of H2O2 into the pulp chamber, intensifying the toxicity of a 35%-H2O2 bleaching gel to pulp cells. (AU)

• Processo FAPESP: 13/23520-0 - Bioatividade de scaffolds experimentais a base de quitosana e colágeno sobre cultura de células pulpares humanas
• Beneficiário: Diana Gabriela Soares dos Passos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado