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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rapid Detection of ACTG- and AK-Toxins in Alternaria alternata by LC-ESI-MS/MS Analysis and Antifungal Properties of Citrus Compounds

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Autor(es):
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Kátia R. Prieto ; Lívia S. de Medeiros ; Marsele M. Isidoro ; Leonardo Toffano ; Maria Fátima G. F. da Silva ; João B. Fernandes ; Paulo C. Vieira ; Moacir R. Forim ; Edson Rodrigues-Filho ; Rodrigo M. Stuart ; Marcos A. Machado
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Journal of the Brazilian Chemical Society; v. 27, n. 8, p. 1493-1505, Ago. 2016.
Resumo

A methanol-soluble fraction of the dichloromethane extract from the culture broth of A. alternata AT4303 strain afforded the following five known mycotoxins: alternariol, alternariol monomethyl ether, altenusin, altenuene and altertoxin I. A hexane-soluble fraction of the dichloromethane extract yielded the steroids ergosterol and ergosterol peroxide, and alternariol monomethyl ether. The concentrated ethyl acetate extract afforded the nucleoside uridine, uracil and inosine. The micro-extracts obtained from this strain in solid media were analyzed using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and the following five known toxins were identified: ACTG-C, D, E and F and AK-toxin II. The major toxins produced by the tangerine pathotype ACT-toxins appear to be absent in strain AT4303. However, the low concentration of ACT-toxins appears to be responsible for their lack of detection in this study. Some compounds isolated from citrus plants were tested for in vitro activity against this strain. The results indicated that 17.3 µM apigenin-7-O-rutinoside inhibited by 80% conidial germination and appressorium development. (AU)

Processo FAPESP: 12/25299-6 - Estudos integrados para o controle de formigas cortadeiras
Beneficiário:João Batista Fernandes
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/57859-5 - Controle biorracional de insetos pragas
Beneficiário:Maria Fátima das Graças Fernandes da Silva
Linha de fomento: Auxílio à Pesquisa - Temático