Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

Autor(es)

Gabriella Dias Arena ^[1] ; Pedro Luis Ramos-González ; Maria Andréia Nunes ^[3] ; Camila Chabi Jesus ; Renata Faier Calegario ^[5] ; Elliot Watanabe Kitajima ^[6] ; Valdenice Moreira Novelli ^[7] ; Juliana Freitas-Astúa

Afiliação do(s) autor(es)

^[1] Agronomic Institute/Sylvio Moreira Citrus Research Center - Brasil

^[3] Agronomic Institute/Sylvio Moreira Citrus Research Center - Brasil

^[5] Federal University of Paraná - Brasil

^[6] University of São Paulo/ESALQ. Dept. of Plant Pathology and Nematology - Brasil

^[7] Agronomic Institute/Sylvio Moreira Citrus Research Center - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Scientia Agricola; v. 74, n. 1, p. 85-89, 2017-02-00.

Resumo

ABSTRACT: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. (AU)

• Processo FAPESP: 12/18771-0 - Análise da expressão gênica de planta-modelo em resposta à leprose dos citros
• Beneficiário: Gabriella Dias Arena
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/08458-9 - Vírus de plantas transmitidos por Brevipalpus (Acari: Tenuipalpidae) - VTB: levantamento, identificação, caracterização molecular, filogenia; relações vírus/vetor/hospedeira; biologia, taxonomia e manejo do vetor
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/00366-8 - Arabidopsis thaliana e Citrus leprosis virus-C: um modelo para o estudo da interação planta-patógeno
• Beneficiário: Gabriella Dias Arena
• Modalidade de apoio: Bolsas no Brasil - Doutorado