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Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

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Autor(es):
Gabriella Dias Arena ; Pedro Luis Ramos-González ; Maria Andréia Nunes ; Camila Chabi Jesus ; Renata Faier Calegario ; Elliot Watanabe Kitajima ; Valdenice Moreira Novelli ; Juliana Freitas-Astúa
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Scientia Agricola; v. 74, n. 1, p. 85-89, Fev. 2017.
Citações Web of Science: 4
Resumo

ABSTRACT: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. (AU)

Processo FAPESP: 14/08458-9 - Vírus de plantas transmitidos por Brevipalpus (Acari: Tenuipalpidae) - VTB: levantamento, identificação, caracterização molecular, filogenia; relações vírus/vetor/hospedeira; biologia, taxonomia e manejo do vetor
Beneficiário:Elliot Watanabe Kitajima
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/00366-8 - Arabidopsis thaliana e Citrus leprosis virus-C: um modelo para o estudo da interação planta-patógeno
Beneficiário:Gabriella Dias Arena
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/18771-0 - Análise da expressão gênica de planta-modelo em resposta à leprose dos citros
Beneficiário:Gabriella Dias Arena
Linha de fomento: Bolsas no Brasil - Mestrado