Maternal vitamin B-6 deficient or supplemented diets on expression of genes related to GABAergic, serotonergic, or glutamatergic pathways in hippocampus of rat dams and their offspring

Autor(es)

Almeida, Mara Ribeiro ; Mabasa, Lawrence ; Crane, Courtney ; Park, Chung S. ; Venancio, Vinicius Paula ; Pires Bianchi, Maria Lourdes ; Greggi Antunes, Lusania Maria

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR NUTRITION & FOOD RESEARCH; v. 60, n. 7, p. 1615-1624, JUL 2016.
• Citações Web of Science: 2

Resumo

ScopeVitamin B-6 plays crucial roles on brain development and its maternal deficiency impacts the gamma-aminobutyric acid (GABA)ergic, serotonergic, glutamatergic, and dopaminergic systems in offspring. However, the molecular mechanisms underlying these neurological changes are not well understood. Thus, we aimed at evaluating which components of those neurotransmitter metabolism and signaling pathways can be modulated by maternal vitamin B-6-deficient or B-6-supplementated diets in the hippocampus of rat dams and their offspring. Methods and resultsFemale Wistar rats were fed three different diets: control (6 mg vitamin B-6/kg), supplemented (30 mg vitamin B-6/kg) or deficient diet (0 mg vitamin B-6/kg), from 4 weeks before pregnancy through lactation. Newborn pups (10 days old) from rat dams fed vitamin B-6-deficient diet presented hyperhomocysteinemia and had a significant increase in mRNA levels of glutamate decarboxylase 1 (Gad1), fibroblast growth factor 2 (Fgf2), and glutamate-ammonia ligase (Glul), while glutaminase (Gls) and tryptophan hydroxylase 1 (Tph1) mRNAs were downregulated. Vitamin B-6 supplementation or deficiency did not change hippocampal global DNA methylation. ConclusionA maternal vitamin B-6-deficient diet affects the expression of genes related to GABA, glutamate, and serotonin metabolisms in offspring by regulating Gad1, Glul, Gls, and Tph1 mRNA expression. (AU)

• Processo FAPESP: 12/20441-9 - Avaliação da instabilidade cromossômica e da expressão de mRNA de genes relacionados com a neurogênese e o metabolismo de neurotransmissores na prole de ratas submetidas a dietas deficiente ou suplementada com vitamina B6
• Beneficiário: Mara Ribeiro de Almeida
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/01288-0 - Efeito da vitamina B6 da dieta materna sobre a modulação da expressão gênica no cérebro de ratas e de seus descendentes
• Beneficiário: Mara Ribeiro de Almeida
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado