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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of different anesthetic agents on left ventricular systolic function assessed by echocardiography in hamsters

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Autor(es):
Tanaka, D. M. ; Romano, M. M. D. ; Carvalho, E. E. V. ; Oliveira, L. F. L. ; Souza, H. C. D. ; Maciel, B. C. ; Salgado, H. C. ; Fazan-Junior, R. ; Simoes, M. V.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 49, n. 10 2016.
Citações Web of Science: 2
Resumo

Determination of left ventricular ejection fraction (LVEF) using in vivo imaging is the cardiac functional parameter most frequently employed in preclinical research. However, there is considerable conflict regarding the effects of anesthetic agents on LVEF. This study aimed at assessing the effects of various anesthetic agents on LVEF in hamsters using transthoracic echocardiography. Twelve female hamsters were submitted to echocardiography imaging separated by 1-week intervals under the following conditions: 1) conscious animals, 2) animals anesthetized with isoflurane (inhaled ISO, 3 L/min), 3) animals anesthetized with thiopental (TP, 50 mg/kg, intraperitoneal), and 4) animals anesthetized with 100 mg/kg ketamine plus 10 mg/kg xylazine injected intramuscularly (K/X). LVEF obtained under the effect of anesthetics (ISO=62.2 +/- 3.1%, TP=66.2 +/- 2.7% and K/X=75.8 +/- 1.6%) was significantly lower than that obtained in conscious animals (87.5 +/- 1.7%, P<0.0001). The K/X combination elicited significantly higher LVEF values compared to ISO (P<0.001) and TP (P<0.05). K/X was associated with a lower dispersion of individual LVEF values compared to the other anesthetics. Under K/X, the left ventricular end diastolic diameter (LVdD) was increased (0.60 +/- 0.01 cm) compared to conscious animals (0.41 +/- 0.02 cm), ISO (0.51 +/- 0.02 cm), and TP (0.55 +/- 0.01 cm), P<0.0001. The heart rate observed with K/X was significantly lower than in the remaining conditions. These results indicate that the K/X combination may be the best anesthetic option for the in vivo assessment of cardiac systolic function in hamsters, being associated with a lower LVEF reduction compared to the other agents and showing values closer to those of conscious animals with a lower dispersion of results. (AU)

Processo FAPESP: 14/07722-4 - Estudo do efeito de agente vasodilatador da microcirculação coronariana sobre a perfusão miocárdica e a disfunção ventricular esquerda em modelo de cardiomiopatia chagásica crônica em hamsters
Beneficiário:Denise Mayumi Tanaka
Linha de fomento: Bolsas no Brasil - Mestrado