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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Transmembrane Protein-Based Electrochemical Biosensor for Adiponectin Hormone Quantification

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Autor(es):
Brazaca, Lais C. ; Janegitz, Bruno C. ; Cancino-Bernardi, Juliana ; Zucolotto, Valtencir
Número total de Autores: 4
Tipo de documento: Artigo Científico
Fonte: CHEMELECTROCHEM; v. 3, n. 6, p. 1006-1011, JUN 2016.
Citações Web of Science: 4
Resumo

Decreased adiponectin hormone secretion occurs 10-20 years before chronic hyperglycemia, and is a great indicator of further development of type 2 diabetes. Here, we developed an electrochemical biosensor for adiponectin quantification based on specific transmembrane receptors (AdipoR1 and AdipoR2). Under optimized conditions, cyclic-voltammetry-based adiponectin biosensors displayed linearity (R-2 = 0.992) in a wide range of concentrations (0.025-0.75 mu mol L-1), including typical human serum levels, with a detection limit of 7.0 nmol L-1 and reproducibility of 1.7%, n = 3. Electrochemical impedance spectroscopy results showed a similar behavior, with a linear increase in charge-transfer resistance (R-2 = 0.971) from 0.025 to 1.00 mu mol L-1, a detection limit of 7.0 nmol L-1, and reproducibility of 5.6 %, n = 3. Furthermore, the analytical results obtained by using the biosensor in human serum samples were in agreement with those obtained by using the enzyme-linked immunosorbent assay (ELISA) at a 95% confidence level. It is expected the proposed device increases the number of predictive type2 diabetes diagnostics, promoting early treatment and patient awareness about the precautions to be taken. (AU)

Processo FAPESP: 15/19099-2 - Desenvolvimento de sensores e biossensores eletroquímicos para diversos fins analíticos
Beneficiário:Bruno Campos Janegitz
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/10608-6 - Desenvolvimento de um sistema para o diagnóstico precoce do diabetes mellitus tipo 2
Beneficiário:Laís Canniatti Brazaca
Linha de fomento: Bolsas no Brasil - Mestrado