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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation

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Pedroso Sakoda, Camila Pivari ; de Toledo, Alessandra Choqueta ; Perini, Adenir ; Pinheiro, Nathalia Montouro ; Hiyane, Meire Ioshie ; Grecco, Simone dos Santos ; Lopes Calvo Tiberio, Iolanda de Fatima ; Saraiva Camara, Niels Olsen ; Martins, Milton de Arruda ; Ghilardi Lago, Joao Henrique ; Righetti, Renato Fraga ; Prado, Carla Maximo
Número total de Autores: 12
Tipo de documento: Artigo Científico
Fonte: ACTA HISTOCHEMICA; v. 118, n. 6, p. 615-624, 2016.
Citações Web of Science: 6
Resumo

Background and purpose: Asthma is a disease of high prevalence and morbidity that generates high costs in hospitalization and treatment. Although the airway is involved in the physiopathology of asthma, there is also evidence of the importance of vascular and lung parenchyma inflammation and remodeling, which can contribute to the functional pulmonary alterations observed in asthmatic patients. Our aim was to evaluate treatment using sakuranetin, a flavone isolated from the twigs of Baccharis retusa (Asteraceae), on vascular and lung parenchyma alterations in an experimental murine model of asthma. Methods: Male BALB/c mice were subjected to a sensitization protocol with ovalbumin for 30 days and were treated with or without sakuranetin (20 mg/kg/mice) or dexamethasone (5 mg/kg/mice); then, the lungs were collected for histopathological analysis. We evaluated extracellular matrix remodeling (collagen and elastic fibers), inflammation (eosinophils and NF-kB) and oxidative stress (8-isoprostane) in the pulmonary vessels and lung parenchyma. The thickness of the vascular wall was quantified, as well as the vascular endothelial growth factor (VEGF) levels. Results: We demonstrated that sakuranetin reduced the number of eosinophils and elastic fibers in both the pulmonary vessels and the lung parenchyma, probably due to a reduction of oxidative stress and of the transcription factor NF-kB and VEGF levels in the lung. In addition, it reduced the thickness of the pulmonary vascular wall. The treatment had no effect on the collagen fibers. In most of the parameters, the effect of sakuranetin was similar to the dexamethasone effect. Conclusions and implications: Sakuranetin had anti-inflammatory and antioxidant effects, preventing vascular and distal parenchyma changes in this experimental model of asthma. (C) 2016 Elsevier GmbH. All rights reserved. (AU)

Processo FAPESP: 10/14831-3 - Efeitos do tratamento com flavonóides derivado de Baccharis retusa em modelo de inflamação pulmonar alérgica crônica induzida por ovoalbumina em camundongos
Beneficiário:Camila Pivari Pedroso Sakoda
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 08/55359-5 - Avaliação da função e da histopatologia pulmonar em modelo experimental de redução da função colinérgica em camundongos geneticamente modificados
Beneficiário:Carla Máximo Prado
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 14/25689-4 - Efeito do sistema colinérgico na inflamação pulmonar aguda e crônica.
Beneficiário:Carla Máximo Prado
Linha de fomento: Auxílio à Pesquisa - Regular