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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Lacking of estradiol reduces insulin exocytosis from pancreatic beta-cells and increases hepatic insulin degradation

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Santos, Roberta S. ; Batista, Thiago M. ; Camargo, Rafael L. ; Morato, Priscila N. ; Borck, Patricia C. ; Leite, Nayara C. ; Kurauti, Mirian A. ; Wanschel, Amarylis C. B. A. ; Nadal, Angel ; Clegg, Deborah J. ; Carneiro, Everardo M.
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Steroids; v. 114, n. SI, p. 16-24, OCT 2016.
Citações Web of Science: 6
Resumo

Low levels of plasma estrogens are associated with weight-gain, android fat distribution, and a high prevalence of obesity-related comorbidities such as glucose intolerance and type II diabetes. The mechanisms underlying the association between low levels of estrogens and impaired glucose homeostasis are not completely understood. To begin to test this, we used three-month-old female C57BL/6J mice that either underwent ovariectomy (OVX) or received a sham surgery (Sham), and we characterized glucose homeostasis. In a subsequent series of experiments, OVX mice received estradiol treatment (OVX + E-2) or vehicle (OVX) for 6 consecutive days. As has been previously reported, lack of ovarian hormones resulted in dysregulated glucose homeostasis. To begin to explore the mechanisms by which this occurs, we characterized the impact of estrogens on insulin secretion and degradation in these mice. Insulin secretion and plasma insulin levels were lower in OVX mice. OVX mice had lower levels of pancreatic Syntaxin 1-A(Synt-1A) protein, which is involved in insulin extrusion from the pancreas. In the liver, OVX mice had higher levels of insulin-degrading enzyme (IDE) and this was associated with higher insulin clearance. Estradiol treatment improved glucose intolerance in OVX mice and restored insulin secretion, as well as normalized the protein content of pancreatic Synt-1A. The addition of estrogens to OVX mice reduced IDE protein to that of Sham mice. Our data suggest loss of ovarian estradiol following OVX led to impaired glucose homeostasis due to pancreatic beta-cell dysfunction in the exocytosis of insulin, and upregulation of hepatic IDE protein content resulting in lower insulinemia, which was normalized by estradiol replacement. (C) 2016 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 12/50430-9 - Caracterização molecular e funcional da secreção e ação da insulina em camundongos fêmeas obesas menopausadas e suplementadas com taurina
Beneficiário:Roberta de Souza Santos
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
Beneficiário:Licio Augusto Velloso
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 15/05801-7 - Investigação do papel do 17 alfa-estradiol em aumentar a inflamação no sistema nervoso central
Beneficiário:Roberta de Souza Santos
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado