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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Endophytic cultivable bacterial community obtained from the Paullinia cupana seed in Amazonas and Bahia regions and its antagonistic effects against Colletotrichum gloeosporioides

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Autor(es):
Santos e Silva, Maria Carolina ; Polonio, Julio Cesar ; Quecine, Maria Carolina ; de Almeida, Tiago Tognolli ; Bogas, Andrea Cristina ; Pamphile, Joao Alencar ; Pereira, Jose Odair ; Astolfi-Filho, Spartaco ; Azevedo, Joao Lucio
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: Microbial Pathogenesis; v. 98, p. 16-22, SEP 2016.
Citações Web of Science: 7
Resumo

Guarana (Paullinia cupana var. sorbilis) is a plant from the Amazonas region with socio-economic importance. However, guarana production has been increasingly affected by unfavorable conditions resulting from anthracnose, caused by the Colletotrichum fungal genus, which primarily affects mainly the Amazonas region. The aim of the present study was to isolate bacterial endophytes from the seeds of guarana plants obtained from Amazonas region and the Northeast state of Bahia, a region where this disease is not a problem for guarana plantations. The number of bacterial Colony Forming Units (CFU/g seeds) was 2.4 x 10(4) from the Bahia and 2.9 x 10(4) from the Amazonas region. One hundred and two isolated bacteria were evaluated in vitro against the phytopathogenic strain Colletotrichum gloeosporioides L1. These isolates were also analyzed for the enzymatic production of amylase, cellulase, protease, pectinase, lipase and esterase. Approximately 15% of isolates, showing high antagonistic activity, and the production of at least one enzyme were identified through the partial sequencing of 16S rDNA. The genus Bacillus was the most frequently observed, followed by Paenibacillus, Ochrobactrum, Microbacterium and Stenotrophomonas. Proteolytic activity was observed in 24 isolates followed by amylolytic, pectinolytic and cellulolytic activities. No esterase and lipase production was detected. Most of the isolates, showing antagonistic effects against C. gloeosporioides and high enzymatic activities, were isolated from the anthracnose-affected region. A biocontrol method using the endophytes from guarana seeds could be applied in the future, as these bacteria are vertically transferred to guarana seedlings. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/14382-4 - Microrganismos endofíticos cultiváveis e não cultiváveis do guaranazeiro e o controle da antracnose
Beneficiário:Maria Leticia Bonatelli
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/53376-2 - Microrganismos associados ao guaranazeiro com potencial biotecnológico e agrícola, especialmente visando o controle da antracnose
Beneficiário:Elliot Watanabe Kitajima
Linha de fomento: Auxílio à Pesquisa - Regular