MicroRNA expression signatures in lungs of mice infected with Mycobacterium tuberculosis

Autor(es):Mostrar menos -

Malardo, Thiago ; Gardinassi, Luiz Gustavo ; Moreira, Bernardo Pereira ; Padilha, Everton ; Cetrulo Lorenzi, Julio Cesar ; Soares, Luana Silva ; Gembre, Ana Flavia ; Fontoura, Isabela Cardoso ; de Almeida, Luciana Previato ; Ferreira de Miranda Santos, Isabel Kinney ; Silva, Celio Lopes

• Tipo de documento: Artigo Científico
• Fonte: TUBERCULOSIS; v. 101, p. 151-159, DEC 2016.
• Citações Web of Science: 3

Resumo

Tuberculosis (TB) is a major public health concern worldwide; however the factors that account for resistance or susceptibility to disease are not completely understood. Although some studies suggest that the differential expression of miRNAs in peripheral blood of TB patients could be useful as biomarkers of active disease, their involvement during the inflammatory process in lungs of infected individuals is unknown. Here, we evaluated the global expression of miRNAs in the lungs of mice experimentally infected with Mycobacterium tuberculosis on 30 and 60 days post-infection. We observed that several miRNAs were differentially expressed compared to uninfected mice. Furthermore, we verified that the expression of miR-135b, miR-21, miR-155, miR-146a, and miR-146b was significantly altered in distinct leukocyte subsets isolated from lungs of infected mice, while genes potentially targeted by those miRNAs were associated with a diversity of immune related molecular pathways. Importantly, we validated the inhibition of Pellino 1 expression by miR-135b in vitro. Overall, this study contributes to the understanding of the dynamics of miRNA expression in lungs during experimental TB and adds further perspectives into the role of miRNAs on the regulation of immune processes such as leukocyte activation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/25856-8 - O Papel da N-glicosilação da Região Fc de IgG na Patogênese da Leishmaniose Visceral e a Avaliação de Novas Estratégias Terapêuticas
• Beneficiário: Luiz Gustavo Araujo Gardinassi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/04405-8 - Metabolismo do triptofano e resistência a carrapatos
• Beneficiário: Thiago Malardo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/01117-3 - Análise dos microRNAs de células dendríticas provenientes de linfonodos drenantes do pulmão de animais infectados com Mycobacterium tuberculosis estimuladas ou não com mRNAhsp65 ou DNAhsp65
• Beneficiário: Arlete Aparecida Martins Coelho-Castelo
• Modalidade de apoio: Auxílio à Pesquisa - Regular