| Texto completo | |
| Autor(es): Mostrar menos - |
de Souza, Jean Gabriel
;
Morais, Katia L. P.
;
Angles-Cano, Eduardo
;
Boufleur, Pamela
;
de Mello, Evandro Sobroza
;
Maria, Durvanei Augusto
;
Taemi Origassa, Clarice Silvia
;
Zampolli, Hamilton de Campos
;
Saraiva Camara, Niels Olsen
;
Berra, Carolina Maria
;
Bosch, Rosemary Viola
;
Chudzinski-Tavassi, Ana Marisa
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ONCOTARGET; v. 7, n. 38, p. 62255-62266, SEP 20 2016. |
| Citações Web of Science: | 6 |
| Resumo | |
Renal cell carcinoma (RCC), also called kidney cancer or renal adenocarcinoma, is highly resistant to current treatments. It has been previously reported that a Kunitz-type inhibitor domain-containing protein, isolated from the salivary glands of the Amblyomma cajennense tick, triggers apoptosis in murine renal adenocarcinoma cells (Renca) by inhibiting the proteasome and endoplasmic reticulum stress. Of note, Amblyomin-X is the corresponding recombinant protein identified in the cDNA library from A. cajennense salivary glands. Herein, using orthotopic kidney tumors in mice, we demonstrate that Amblyomin-X is able to drastically reduce the incidence of lung metastases by inducing cell cycle arrest and apoptosis. The in vitro assays show that Amblyomin-X is capable of reducing the proliferation rate of Renca cells, promoting cell cycle arrest, and down-regulating the expression of crucial proteins (cyclin D1, Ki67 and Pgp) involved in the aggressiveness and resistance of RCC. Regarding non-tumor cells (NIH3T3), Amblyomin-X produced minor effects in the cyclin D1 levels. Interestingly, observing the image assays, the fluorescence-labelled Amblyomin-X was indeed detected in the tumor stroma whereas in healthy animals it was rapidly metabolized and excreted. Taken the findings together, Amblyomin-X can be considered as a potential anti-RCC drug candidate. (AU) | |
| Processo FAPESP: | 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular |
| Beneficiário: | Hugo Aguirre Armelin |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival |
| Beneficiário: | Ana Marisa Chudzinski-Tavassi |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia |
| Processo FAPESP: | 12/02270-2 - Novos mecanismos celulares, moleculares e imunológicos das lesões renais agudas e crônicas: busca por novas estratégias terapêuticas |
| Beneficiário: | Niels Olsen Saraiva Câmara |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 10/52669-3 - Avaliação do mecanismo de ação pró-apoptótica do Amblyomin-X |
| Beneficiário: | Ana Marisa Chudzinski-Tavassi |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/06944-8 - Efeito do Amblyomin-X na interface do sistema imune e hemostático durante a progressão do tumor RENCA ( carcinoma renal murino): in vivo e in vitro |
| Beneficiário: | Jean Gabriel de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 10/07958-7 - Avaliação do mecanismo de ação pró-apoptótica do amblyomin-x |
| Beneficiário: | Kátia Luciano Pereira Morais |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |