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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Systemic immunization with rPotD reduces Streptococcus pneumoniae nasopharyngeal colonization in mice

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Converso, T. R. ; Goulart, C. ; Rodriguez, D. ; Darrieux, M. ; Leite, L. C. C.
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: Vaccine; v. 35, n. 1, p. 149-155, JAN 3 2017.
Citações Web of Science: 6

Streptococcus pneumoniae (pneumococcus) is a human pathogen that can cause otitis media, pneumonia and, in severe cases, meningitis and bacteremia. The pneumococcus expresses PotD, a protein belonging to the polyamines transporter complex called PotABCD. PotD is a membrane-associated protein that binds polyamines and has been shown to be important for virulence. In this work we demonstrate that subcutaneous immunization with rPotD reduces the bacterial load in the nasal tissue of mice, following intranasal challenge with a type 6B pneumococcus. The protective effect correlated with the induction of high levels of antibodies in the immunized group; the antibodies were able to increase bacterial phagocytosis by mouse peritoneal cells. The cellular immune response was characterized by the production of gamma-interferon, IL-2 and IL-17 by splenocytes and nitric oxide by peritoneal cells of immunized mice, upon stimulation with rPotD. Taken together our results suggest that PotD is a promising candidate to be included in a protein based pneumococcal vaccine, able to induce phagocytic antibodies, a Th1 cellular immune response and production of IL-17, reducing nasopharyngeal colonization, the main event responsible for transmission of pneumococci in humans. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 12/04286-3 - O estudo do transportador de poliaminas, PotD, como antígeno vacinal contra Streptococcus pneumoniae
Beneficiário:Thiago Rojas Converso
Linha de fomento: Bolsas no Brasil - Doutorado Direto