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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dantrolene improves in vitro structural changes induced by serum from Trypanosoma cruzi-infected mice

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Autor(es):
Malvestio, Lygia M. ; Celes, Mara Rubia N. ; Jelicks, Linda A. ; Tanowitz, Herbert B. ; Prado, Cibele M.
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: Parasitology Research; v. 116, n. 1, p. 429-433, JAN 2017.
Citações Web of Science: 2
Resumo

Dystrophin, an important protein of the dystrophin-glycoprotein complex, has been implicated in the pathogenesis of experimental Chagas disease. It is important for the maintenance of cell shape and contraction force transmission. Dystrophin loss has been related to end-stage cardiac myopathies and proposed as a common route for myocardial dysfunction and progression to advanced heart failure. Evidence suggests that calpains, calcium-dependent proteases, digest dystrophin when the calcium concentration is compatible with their activation. The objective of this in vitro study was to test the hypothesis that dantrolene, a calcium channel blocker, improves structural changes induced by serum from Trypanosoma cruzi-infected mice. Cultured neonatal cardiac myocytes were incubated with serum from T. cruzi-infected mice and treated with dantrolene for 24 h. Immunofluorescence and immunoblotting were performed to evaluate dystrophin and calpain-1 protein expression. The levels of dystrophin decreased 13% and calpain increased 17% after incubation of cultured neonatal cardiac myocytes with serum from T. cruzi-infected mice. The treatment with dantrolene restored the dystrophin and calpain levels near control levels. Our results demonstrate that alterations in calcium homeostasis in cardiac myocytes are responsible, in part, for cardiac structural changes in experimentally induced T. cruzi myocarditis and that calpain inhibitors may be beneficial in Chagasic heart disease. (AU)

Processo FAPESP: 10/19216-5 - Hipertensão arterial, hipertrofia e falência cardíaca e complexo de glicoproteínas associadas à distrofina
Beneficiário:Cibele Maria Prado Zinni
Linha de fomento: Bolsas no Brasil - Apoio a Jovens Pesquisadores
Processo FAPESP: 09/17787-8 - Hipertensão arterial, hipertrofia e falência cardíaca e complexo de glicoproteínas associadas à distrofina.
Beneficiário:Cibele Maria Prado Zinni
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 09/53544-2 - Avaliação in vitro da expressão de distrofina em cardiomiócitos submetidos a diferentes estímulos
Beneficiário:Marcos Antonio Rossi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/18629-4 - O papel do cálcio, da calpaína, de citocinas pró-inflamatórias e de hipóxia na expressão de distrofina em cardiomiócitos: estudo in vitro
Beneficiário:Lygia Maria Mouri Malvestio
Linha de fomento: Bolsas no Brasil - Doutorado Direto