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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Gene delivery and immunomodulatory effects of plasmid DNA associated with Branched Amphiphilic Peptide Capsules

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Autor(es):
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Avila, L. A. ; Aps, L. R. M. M. ; Ploscariu, N. ; Sukthankar, P. ; Guo, R. ; Wilkinson, K. E. ; Games, P. ; Szoszkiewicz, R. ; Alves, R. P. S. ; Diniz, M. O. ; Fang, Y. ; Ferreira, L. C. S. ; Tomich, J. M.
Número total de Autores: 13
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF CONTROLLED RELEASE; v. 241, p. 15-24, NOV 10 2016.
Citações Web of Science: 8
Resumo

We recently reported on a new class of branched amphiphilic peptides that associate with double stranded DNA and promote in vitro transfection of eukaryotic cells. In the present study, we tested a different formulation in which plasmid DNA associates with the surface of preformed 20-30 nm cationic capsules formed through the self-assembly of the two branched amphiphilic peptides. Under these conditions, the negatively charged DNA interacts with the cationic surface of the Branched Amphiphilic Peptide Capsules (BAPCs) through numerous electrostatic interactions generating peptide-DNA complexeswith sizes ranging from50 to 250 nm. The BAPCs-DNA nanoparticles are capable of delivering plasmid DNA of different size into cells in culture, yielding high transfection rates and minimal cytotoxicity. Furthermore, BAPCs were tested for in vivo delivery of a DNA vaccine previously designed to activate immune responses and capable of controlling tumors induced by type 16 human papilloma virus (HPV-16). The BAPCs-DNA nanoparticles enhanced the vaccine-induced antitumor protection and promoted activation of murine dendritic cells without significant toxic effects. These results indicate that branched amphiphilic oligo-peptides nanoparticles represent a new and promising nonviral DNA/gene delivery approach endowing immunomodulatory properties for DNA vaccines. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/15360-2 - Novas fronteiras terapêuticas contra tumores causados pelo vírus do papiloma humano (HPV): avaliação experimental da associação da quimioterapia com estratégias vacinais
Beneficiário:Luana Raposo de Melo Moraes Aps
Linha de fomento: Bolsas no Brasil - Doutorado