Short communication: Outbreak of methicillin-resistant Staphylococcus aureus (MRSA)-associated mastitis in a closed dairy herd

Autor(es)

Guimaraes, F. F. ; Manzi, M. P. ; Joaquim, S. F. ; Richini-Pereira, V. B. ; Langoni, H.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DAIRY SCIENCE; v. 100, n. 1, p. 726-730, JAN 2017.
• Citações Web of Science: 12

Resumo

Cows are probably the main source of contamination of raw milk with Staphylococcus aureus. Mammary glands with subclinical mastitis can shed large numbers of Staph. aureus in milk. Because of the risk of this pathogen to human health as well as animal health, the aim of this paper was to describe an outbreak of mastitis caused by methicillin-resistant Staph. aureus (MRSA), oxacillin-susceptible mecA-positive Staph. aureus (OS-MRSA), and methicillin-susceptible Staph. aureus (MSSA) on a dairy farm. Milk samples were obtained from all quarters, showing an elevated somatic cell count by the California Mastitis Test. The isolates were identified by phenotypic and genotypic methods. Staphylococcus spp. were isolated from 53% (61/115) of the milk samples, with 60 isolates identified as Staph. aureus (98.4%) and 1 isolate identified as Staphylococcus epidermidis (1.6%). The presence of the mecA gene was verified in 48.3% of Staph. aureus isolates. Of the Staph. aureus isolates, 23.3% were MRSA and 25.0% were OS-MRSA. The total of mastitis cases infected with MRSA was 12.2%. The detection of this large percentage of mastitis cases caused by MRSA and OS-MRSA is of great concern for the animals' health, because 13-lactams are still the most important antimicrobials used to treat mastitis. In addition, Staph. aureus isolates causing bovine mastitis represent a public health risk. (AU)

• Processo FAPESP: 08/11614-1 - PAPEL DOS ESTAFILOCOCOS COAGULASE NEGATIVA NAS MASTITES BOVINAS: PERFIL DE SENSIBILIDADE MICROBIANA, PESQUISA DE GENE mecA DE RESISTÊNCIA À METICILINA E DETECÇÃO MOLECULAR DE GENES CODIFICADORES DE ENTEROTOXINAS
• Beneficiário: Helio Langoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/21323-7 - Pesquisa de genes codificadores de enterotoxinas estafilocócicas e gene vanA de resistência à vancomicina: detecção da expressão gênica em estafilococos coagulase positiva e negativa nas mastites bovinas
• Beneficiário: Helio Langoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/08780-7 - Papel dos estafilococos coagulase negativa nas mastites bovinas: perfil de sensibilidade microbiana, pesquisa de gene mecA de resistência à meticilina e detecção molecular de genes codificadores de enterotoxinas
• Beneficiário: Felipe de Freitas Guimarães
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/21142-2 - Pesquisa de genes codificadores de enterotoxinas estafilocócicas e gene vanA de resistência à vancomicina: detecção da expressão gênica em estafilococos coagulase positiva e negativa nas mastites bovinas
• Beneficiário: Felipe de Freitas Guimarães
• Modalidade de apoio: Bolsas no Brasil - Doutorado