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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Transgenic sugarcane overexpressing CaneCPI-1 negatively affects the growth and development of the sugarcane weevil Sphenophorus levis

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Autor(es):
Schneider, Vanessa Karine ; Soares-Costa, Andrea ; Chakravarthi, Mohan ; Ribeiro, Carolina ; Chabregas, Sabrina Moutinho ; Falco, Maria Cristina ; Henrique-Silva, Flavio
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Plant Cell Reports; v. 36, n. 1, p. 193-201, JAN 2017.
Citações Web of Science: 4
Resumo

Transgenic sugarcane expressing CaneCPI-1 exhibits resistance to Sphenophorus levis larvae. Transgenic plants have widely been used to improve resistance against insect attack. Sugarcane is an economically important crop; however, great losses are caused by insect attack. Sphenophorus levis is a sugarcane weevil that digs tunnels in the stem base, leading to the destruction of the crop. This insect is controlled inefficiently by chemical insecticides. Transgenic plants expressing peptidase inhibitors represent an important strategy for impairing insect growth and development. Knowledge of the major peptidase group present in the insect gut is critical when choosing the most effective inhibitor. S. levis larvae use cysteine peptidases as their major digestive enzymes, primarily cathepsin L-like activity. In this study, we developed transgenic sugarcane plants that overexpress sugarcane cysteine peptidase inhibitor 1 (CaneCPI-1) and assessed their potential through feeding bioassays with S. levis larvae. Cystatin overexpression in the transgenic plants was evaluated using semi-quantitative RT-PCR, RT-qPCR, and immunoblot assays. A 50% reduction of the average weight was observed in larvae that fed on transgenic plants in comparison to larvae that fed on non-transgenic plants. In addition, transgenic sugarcane exhibited less damage caused by larval attack than the controls. Our results suggest that the overexpression of CaneCPI-1 in sugarcane is a promising strategy for improving resistance against this insect. (AU)

Processo FAPESP: 05/59833-5 - Desenvolvimento de canas-de-açúcar transgênicas superexpressando cistatinas visando o aumento da resistência a insetos
Beneficiário:Andrea Soares da Costa Fuentes
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 98/14138-2 - Center for Structural Molecular Biotechnology
Beneficiário:Glaucius Oliva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 13/05370-0 - Desenvolvimento de linhagens de cana-de-açúcar transgênica superexpressando inibidores de peptidases visando resistência ao inseto Migdolus fryanus
Beneficiário:Vanessa Karine Schneider
Linha de fomento: Bolsas no Brasil - Doutorado Direto