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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Atypical enteropathogenic Escherichia coli as aetiologic agents of sporadic and outbreak-associated diarrhoea in Brazil

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Autor(es):
Vieira, Melissa A. ; dos Santos, Luis F. ; Dias, Regiane C. B. ; Camargo, Carlos H. ; Pinheiro, Sandra R. S. ; Gomes, Tania A. T. ; Hernandes, Rodrigo T.
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Journal of Medical Microbiology; v. 65, n. 9, p. 998-1006, SEP 2016.
Citações Web of Science: 13
Resumo

Enteropathogenic Escherichia coli (EPEC) are important agents of diarrhoea in industrialized as well as developing countries, such as Brazil. The hallmark of EPEC pathogenesis is the establishment of attaching and effacing lesions in enterocytes, in which pedestal-like structures are formed underneath adherent bacteria. EPEC are divided into two subgroups, typical (tEPEC) and atypical (aEPEC), based on the presence of the EPEC adherence factor plasmid in tEPEC and its absence in aEPEC. This study was designed to characterize 82 aEPEC isolates obtained from stool samples of diarrhoeic patients during 2012 and 2013 in Brazil. The majority of the aEPEC were assigned to the phylo-group B1 (48.8 %), and intimin subtypes theta (20.7 %), beta 1 (9.7 %) and lambda (9.7 %) were the most prevalent among the isolates. The nleB and nleE genes were concomitantly detected in 32.9% of the isolates, demonstrating the occurrence of the pathogenicity island O122 among them. The O157-plasmid genes (ehxA and/or espP) were detected in 7.3% of the isolates, suggesting that some aEPEC could be derived from Shiga-toxin-producing E. coli that lost the stx genes while trafficking in the host. PFGE of 14 aEPEC of serotypes O2:H16, O33:H34, O39:H9, O108:H- and ONT:H19 isolated from five distinct outbreaks showed serotype-specific PFGE clusters, indicating a high degree of similarity among the isolates from the same event, thus highlighting these serotypes as potential aetiologic agents of diarrhoeal outbreaks in Brazil. (AU)

Processo FAPESP: 12/19077-0 - Prevalência e caracterização fenotípica e molecular de amostras de Escherichia coli diarreiogênica isoladas de crianças com e sem diarréia na cidade Botucatu, São Paulo
Beneficiário:Rodrigo Tavanelli Hernandes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/23414-5 - Caracterização e análise do perfil de virulência de Amostras de Escherichia coli enteropatogênica atípica isoladas de surtos e casos esporádicos de diarréia em diferentes regiões geográficas do Brasil
Beneficiário:Melissa Arruda Vieira
Linha de fomento: Bolsas no Brasil - Mestrado