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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Iron Content Affects Lipogenic Gene Expression in the Muscle of Nelore Beef Cattle

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Autor(es):
da Silva Diniz, Wellison Jarles ; Coutinho, Luiz Lehmann ; Tizioto, Polyana Cristine ; Mello Cesar, Aline Silva ; Gromboni, Caio Fernando ; Araujo Nogueira, Ana Rita ; Neubern de Oliveira, Priscila Silva ; de Souza, Marcela Maria ; de Almeida Regitano, Luciana Correia
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 11, n. 8 AUG 17 2016.
Citações Web of Science: 5
Resumo

Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to investigate the likely role of these DE genes in biological processes underlying beef quality parameters. Samples for RNA extraction for sequencing and iron, copper, manganese, and zinc determination were collected from LD muscles at slaughter. Eight Nelore steers, with extreme genomic estimated breeding values for iron content (Fe-GEBV), were selected from a reference population of 373 animals. From the 49 annotated DE genes (FDR<0.05) found between the two groups, 18 were up-regulated and 31 down-regulated for the animals in the low Fe-GEBV group. The functional enrichment analyses identified several biological processes, such as lipid transport and metabolism, and cell growth. Lipid metabolism was the main pathway observed in the analysis of metabolic and canonical signaling pathways for the genes identified as DE, including the genes FASN, FABP4, and THRSP, which are functional candidates for beef quality, suggesting reduced lipogenic activities with lower iron content. Our results indicate metabolic pathways that are partially influenced by iron, contributing to a better understanding of its participation in skeletal muscle physiology. (AU)

Processo FAPESP: 12/23638-8 - Bases moleculares da qualidade da carne em bovinos da raça Nelore
Beneficiário:Luciana Correia de Almeida Regitano
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/12652-5 - Expressão gênica diferencial associada ao conteúdo de ferro no músculo em animais Nelore
Beneficiário:Wellison Jarles da Silva Diniz
Linha de fomento: Bolsas no Brasil - Mestrado