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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle

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Autor(es):
Castilho, A. C. S. ; Price, C. A. ; Dalanezi, F. ; Ereno, R. L. ; Machado, M. F. ; Barros, C. M. ; Gasperin, B. G. ; Goncalves, P. B. D. ; Buratini, J.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 29, n. 2, p. 234-243, 2017.
Citações Web of Science: 1
Resumo

There is evidence that regulation of follicle selection in cattle involves locally produced growth factors. In the present study, we investigated the expression of members of the fibroblast growth factor (FGF) 7 family during follicle deviation. The largest and second largest follicles were recovered during the second day of a synchronised follicle wave and the future dominant and future subordinate follicles were identified based on diameter and cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1) mRNA levels in granulosa cells. Theca cells of the future dominant follicle contained less mRNA encoding FGF7 and FGF10 compared with those from the future subordinate follicle 2.5 days after ovulation, before a significant difference between the diameters of the future dominant and future subordinate follicles could be observed, but FGF22 mRNA levels did not change. Levels of mRNA encoding FGF receptors FGFR1B and FGFR2B in theca and granulosa cells, respectively, were lower in the future dominant follicle compared with the future subordinate follicle. Addition of FGF10 to granulosa cells in vitro significantly decreased oestradiol secretion, as well as CYP19A1, FSH receptor (FSHR) and insulin-like growth factor 1 receptor (IGF1R) mRNA abundance, whereas FGF22 had no effect. We conclude that FGF10 and FGFR2B expression is increased in the future subordinate follicle before morphological deviation, which may contribute to follicle selection. (AU)

Processo FAPESP: 08/57365-2 - Expressão e função de fatores de crescimento fibroblástico (FGF) e seus receptores em folículos antrais bovinos
Beneficiário:José Buratini Junior
Linha de fomento: Auxílio à Pesquisa - Regular