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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antithrombotic activity of Batroxase, a metalloprotease from Bothrops atrox venom, in a model of venous thrombosis

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Autor(es):
Jacob-Ferreira, Anna L. ; Menaldo, Danilo L. ; Sartim, Marco A. ; Riul, Thalita B. ; Dias-Baruffi, Marcelo ; Sampaio, Suely V.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 95, p. 263-267, FEB 2017.
Citações Web of Science: 6
Resumo

Background: Snake venoms are great sources of bioactive molecules, which may be used as models for new drugs. Toxins that interfere in hemostasis have received considerable attention over the years. Objectives: This study aimed at the evaluation of the antithrombotic activity of Batroxase, a P-I metalloprotease from Bothrops atrox venom, in an animal model of venous thrombosis. Methods: The antithrombotic activity of Batroxase was tested in vivo in a model based on two factors of the Virchow's Triad: blood flow alterations (partial stenosis of the inferior vena cava), and vessel wall injury (10% ferric chloride for 5 min), in comparison with sodium heparin (positive control) and saline (negative control). Bleeding/clotting time was assessed by a tail bleeding assay. The immunogenicity of Batroxase was also analyzed. Results: Batroxase (12 mg/kg) reduced thrombus formation in 81%, similarly to heparin (100 U/kg), which reduced it in 85% in comparison with the saline group. Both Batroxase and heparin increased bleeding/clotting time in approximately 3 fold. Immunizations of rabbits with Batroxase do not result in detectable levels of antibodies against this metalloprotease. Conclusion: Batroxase presents antithrombotic activity in vivo. Moreover, its lack of immunogenicity increases the interest on its possible therapeutic potential over thrombogenic disorders. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 12/21569-9 - Atividades antitrombóticas e TROMBOLÍTICAS da BATROXASE, uma metaloprotease purificada do veneno da cobra Bothrops atrox, em modelos experimentais animais
Beneficiário:Anna Laura Bechara Jacob Ferreira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
Beneficiário:Suely Vilela
Linha de fomento: Auxílio à Pesquisa - Temático