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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Application of aluminum chloride phthalocyanine-loaded solid lipid nanoparticles for photodynamic inactivation of melanoma cells

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Autor(es):
Goto, Patricia L. ; Siqueira-Moura, Marigilson P. ; Tedesco, Antonio C.
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: International Journal of Pharmaceutics; v. 518, n. 1-2, p. 228-241, FEB 25 2017.
Citações Web of Science: 12
Resumo

Cutaneous melanoma is the most aggressive skin cancer and is particularly resistant to current therapeutic approaches. Photodynamic therapy (PDT) is a well-established photoprocess that is employed to treat some cancers, including non-melanoma skin cancer. Aluminum chloride phthalocyanine (CIAIPc) is used as a photosensitizer in PDT; however, its high hydrophobicity hampers its photodynamic activity under physiological conditions. The aim of this study was to produce solid lipid nanoparticles (SLN) containing CIAIPc using the direct emulsification method. CIAIPc-loaded SLNs (CIAIPc/SLNs) were characterized according to their particle size and distribution, zeta potential, morphology, encapsulation efficiency, stability, and phototoxic action in vitro in B16-F10 melanoma cells. CIAIPc/SLN had a mean diameter between 100 and 200 nm, homogeneous size distribution (polydispersity index <0.3), negative zeta potential, and spherical morphology. The encapsulation efficiency was approximately 100%. The lipid crystallinity was investigated using X-ray diffraction and differential scanning calorimetry and indicated that CIAIPc was integrated into the SLN matrix. The CIAIPc/SLN formulations maintained their physicochemical stability without expelling the drug over a 24-month period. Compared to free CIAIPc, CIAIPc/SLN exerted outstanding phototoxicity effects in vitro against melanoma cells. Therefore, our results demonstrated that the CIAIPc/SLN described in the current study has the potential for use in further preclinical and clinical trials in PDT for melanoma treatment. (C) 2017 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático