Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine

Autor(es)

Siqueira das Neves, Lais Mara ; Marcondes Ferreira Leite, Gabriella de Paula ; Marcolino, Alexandre Marcio ; Pinfildi, Carlos Eduardo ; Garcia, Sergio Britto ; de Araujo, Joao Eduardo ; de Oliveira Guirro, Elaine Caldeira

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 32, n. 2, p. 335-341, FEB 2017.
• Citações Web of Science: 8

Resumo

The aim of this study was to investigate the effect of laser photobiomodulation (PBM) on the viability of the transverse rectus abdominis musculocutaneous (TRAM) flap in rats subjected to the action of nicotine. We evaluated 60 albino Wistar rats, divided into six groups of ten animals. Group 1 (saline) underwent the surgical technique to obtain a TRAM flap; group 2 (laser 830 nm) underwent the surgical technique and was irradiated with a laser 830 nm; group 3 (laser 660 nm) underwent the surgical technique and was irradiated with a laser 660 nm; group 4 was treated with nicotine subcutaneously (2 mg/kg/2x/day/4 weeks) and underwent surgery; group 5 (nicotine + laser 830 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 830 nm; group 6 (nicotine + laser 660 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 660 nm. The application of PBM occurred immediately after surgery and on the two following days. The percentage of necrosis was assessed using the AxioVisionA (R) software. The number of mast cells (toluidine blue staining) was evaluated, and immunohistochemistry was performed to detect vascular endothelial growth factor expression (anti-VEGF-A), fibroblasts (anti-basic FGF), and neoformed vessels (anti-CD34). PBM with a wavelength of 830 nm increased the viability of the TRAM flap, with a smaller area of necrosis, increased number of mast cells, and higher expression of VEGF and CD34. PBM increases the viability of musculocutaneous flaps treated with to nicotine. (AU)

• Processo FAPESP: 13/07732-7 - Laser de baixa intensidade e estimulação elétrica de alta voltagem na viabilidade do retalho musculafasciocutâneo transverso do músculo reto do abdome em ratos submetidos a ação da nicotina
• Beneficiário: Elaine Caldeira de Oliveira Guirro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07602-6 - Laser de baixa intensidade e estimulaçao elétrica de alta voltagem na viabilidade do retalho musculofasciocutâneo transverso do músc em ratos submetidos a ação da nicotina.
• Beneficiário: Lais Mara Siqueira das Neves
• Modalidade de apoio: Bolsas no Brasil - Doutorado