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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

New Sugarcane Microsatellites and Target Region Amplification Polymorphism Primers Designed from Candidate Genes Related to Disease Resistance

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Autor(es):
dos Santos, Fernanda R. C. ; Zucchi, Maria I. ; Park, Jong-Won ; Benatti, Thiago R. ; da Silva, Jorge A. ; Souza, Glaucia M. ; Landell, Marcos G. A. ; Pinto, Luciana R.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: SUGAR TECH; v. 19, n. 2, p. 219-224, APR 2017.
Citações Web of Science: 0
Resumo

Many types of molecular marker systems have been developed to detect and map genomic regions related to disease resistance in many major agronomic crops. However, in sugarcane, molecular marker systems for genetic mapping of genomic regions associated to disease resistance is not yet established mainly due to its complex polyploid genome. In addition, there is a lack of information to develop molecular marker systems (e.g., TRAP and EST-SSR) targeting specific genic regions related to disease resistance. In the present work, a set of 10 EST-SSR primer pairs and 16 fixed TRAP primers designed from sugarcane-derived sequence related to disease resistance are reported. Most of the primers were amplifiable and polymorphic in the sugarcane genotypes tested. In addition, these set of primers were also tested in sorghum, Miscanthus and a cross between sugarcane commercial varieties showing good cross-transferability. This new set of primers, in particular the TRAP ones, will be useful in genetic mapping of genome regions related to disease resistance in sugarcane and related genera. (AU)

Processo FAPESP: 05/55258-6 - Mapeamento genético e identificação de marcadores moleculares genômicos e funcionais associados a características agronômicas de interesse em cana-de-açúcar
Beneficiário:Luciana Rossini Pinto Machado da Silva
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 08/52146-0 - Sugarcane signaling and regulatory networks
Beneficiário:Glaucia Mendes Souza
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático