Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Chimeric viruses between Rocio and West Nile: the role for Rocio prM-E proteins in virulence and inhibition of interferon-alpha/beta signaling

Texto completo
Autor(es):
Amarilla, Alberto A. ; Setoh, Yin X. ; Periasamy, Parthiban ; Peng, Nias Y. ; Pali, Gabor ; Figueiredo, Luiz T. ; Khromykh, Alexander A. ; Aquino, Victor H.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 7, MAR 20 2017.
Citações Web of Science: 7
Resumo

Mosquito-transmitted flavivirus Rocio (ROCV) was responsible for an outbreak of encephalitis in the Ribeira Valley, located in the south coast of Sao Paulo State, Brazil, in 1975-1976. ROCV also causes fatal encephalitis in adult mice. Seroprevalence studies in humans, horses and water buffaloes in different regions of Brazil have suggested that ROCV is still circulating in the country, indicating the risk of re-emergence of this virus. West Nile virus (WNV) is also a mosquito-transmitted encephalitic flavivirus, however, WNV strains circulating in Australia have not been associated with outbreaks of disease in humans and exhibit low virulence in adult mice. To identify viral determinants of ROCV virulence, we have generated reciprocal chimeric viruses between ROCV and the Australian strain of WNV by swapping structural prM and E genes. Chimeric WNV containing ROCV prM-E genes replicated more efficiently than WNV or chimeric ROCV containing WNV prM-E genes in mammalian cells, was as virulent as ROCV in adult mice, and inhibited type I IFN signaling as efficiently as ROCV. The results show that ROCV prM and E proteins are major virulence determinants and identify unexpected function of these proteins in inhibition of type I interferon response. (AU)

Processo FAPESP: 12/11169-3 - Análise da participação da proteína e na neuroinvasividade e neurovirulência do vírus Rocio em modelo animal
Beneficiário:Victor Hugo Aquino Quintana
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/20930-5 - Construção de clones infecciosos de Flavivirus encefalíticos e identificação dos determinantes moleculares na glicoproteína e para infectividade viral
Beneficiário:Alberto Anastacio Amarilla Ortiz
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado