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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cancer cachexia differentially regulates visceral adipose tissue turnover

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Franco, Felipe de Oliveira ; Lopes, Magno Alves ; Henriques, Felipe dos Santos ; das Neves, Rodrigo Xavier ; Bianchi Filho, Cesario ; Batista, Jr., Miguel Luiz
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Journal of Endocrinology; v. 232, n. 3, p. 493-500, MAR 2017.
Citações Web of Science: 5

Cancer cachexia (CC) is a progressive metabolic syndrome that is marked by severe body weight loss. Metabolic disarrangement of fat tissues is a very early event in CC, followed by adipose tissue (AT) atrophy and remodelling. However, there is little information regarding the possible involvement of cellular turnover in this process. Thus, in this study, we evaluated the effect of CC on AT turnover and fibrosis of mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue depots as possible factors that contribute to AT atrophy. CC was induced by a subcutaneous injection of Walker tumour cells (2 x 107) in Wistar rats, and control animals received only saline. The experimental rats were randomly divided into four experimental groups: 0 days, 4 days, 7 days and 14 days after injection. AT turnover was analysed according to the Pref1/Adiponectin ratio of gene expression from the stromal vascular fraction and pro-apoptotic CASPASE3 and CASPASE9 from MEAT and RPAT. Fibrosis was verified according to the total collagen levels and expression of extracellular matrix genes. AT turnover was verified by measurements of lipolytic protein expression. We found that the Pref1/Adiponectin ratio was decreased in RPAT (81.85%, P < 0.05) with no changes in MEAT compared with the respective controls. CASPASE3 and CASPASE9 were activated on day 14 only in RPAT. Collagen was increased on day 7 in RPAT (127%) and MEAT (4.3-fold). The Collagen1A1, Collagen3A1, Mmp2 and Mmp9 mRNA levels were upregulated only in MEAT in CC. Lipid turnover was verified in RPAT and was not modified in CC. We concluded that the results suggest that CC affects RPAT cellular turnover, which may be determinant for RPAT atrophy. (AU)

Processo FAPESP: 15/19259-0 - Repercussões do remodelamento do tecido adiposo durante a síndrome de caquexia em pacientes com câncer gastrointestinal: potencial envolvimento do receptor TLR4 na modulação do browning no TAB
Beneficiário:Miguel Luiz Batista Junior
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/00488-0 - Balanço entre matriz extracelular e apoptose no tecido adiposo branco em ratos com caquexia induzida.
Beneficiário:Felipe de Oliveira Franco
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 10/51078-1 - Bases moleculares da caquexia: adipogênese e remodelagem da matriz extracelular do tecido adiposo branco de pacientes com câncer gastrointestinal
Beneficiário:Miguel Luiz Batista Junior
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores