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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Calorie restriction promotes cardiolipin biosynthesis and distribution between mitochondrial membranes

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Autor(es):
Luevano-Martinez, Luis Alberto ; Forni, Maria Fernanda ; Peloggia, Julia ; Watanabe, Ii-Sei ; Kowaltowski, Alicia J.
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: MECHANISMS OF AGEING AND DEVELOPMENT; v. 162, p. 9-17, MAR 2017.
Citações Web of Science: 7
Resumo

Calorie restriction (CR) has been amply demonstrated to modify mitochondrial function. However, little is known regarding the effects of this dietary regimen on mitochondrial membranes. We isolated phospholipids from rat liver mitochondria from animals on CR or ad libitum diets and found that mitochondria from ad libitum animals present an increased content of lipoperoxides and the content of cardiolipin. Cardiolipin is the main anionic phospholipid present in mitochondrial membranes, and plays a key role in mitochondria( function, signaling and stress response. Expression levels of the enzymes involved in cardiolipin biosynthesis and remodeling were quantified and found to be upregulated in CR animals. Interestingly, when mitochondria( membranes were fractionated, the outer membrane presented a higher content of cardiolipin, indicating a redistribution of this phospholipid mediated by a phospholipid scram blase in CR. This change is associated with Drp1-mediated mitochondrial fragmentation and autophagy. Overall, we find that CR promotes extensive mitochondrial membrane remodeling, decreasing oxidatively damaged lipids, and increasing cardiolipin levels and redistributing cardiolipin. These changes in membrane properties are consistent with and may be causative of changes in mitochondrial morphology, function and turnover previously found to occur in CR. (C) 2017 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 13/04871-6 - Efeitos da restrição calórica sobre a morfologia, dinâmica, bioenergética e estado redox mitocondriais
Beneficiário:Maria Fernanda Pereira de Araújo Demonte Forni
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/04919-9 - Avaliação da resposta adaptativa das membranas mitocondriais em situações de estresse: papel da cardiolipina na plasticidade da membrana, dinâmica e situações patológicas
Beneficiário:Luis Alberto Luevano Martinez
Linha de fomento: Bolsas no Brasil - Pós-Doutorado