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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Collagen-based silver nanoparticles: Study on cell viability, skin permeation, and swelling inhibition

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Cardoso, Vinicius Saura ; Filgueiras, Marcelo de Carvalho ; Dutra, Yago Medeiros ; Gomes Teles, Ramon Handerson ; de Araujo, Alyne Rodrigues ; Primo, Fernando Lucas ; Mafud, Ana Carolina ; Batista, Larissa Fernandes ; Mascarenhas, Yvonne Primerano ; Martinez Paino, Ieda Maria ; Zucolotto, Valtencir ; Tedesco, Antonio Claudio ; Silva, Durcilene Alves ; Leite, Jose Roberto S. A. ; dos Santos, Jr., Jose Ribeiro
Número total de Autores: 15
Tipo de documento: Artigo de Revisão
Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 74, p. 382-388, MAY 1 2017.
Citações Web of Science: 3
Resumo

Collagen is considered the most abundant protein in the animal kingdom, comprising 30% of the total amount of proteins and 6% of the human body by weight Studies that examine the interaction between silver nanoparticles and proteins have been highlighted in the literature in order to understand the stability of the nanoparticle system, the effects observed in biological systems, and the appearance of new chemical pharmaceutical products. The objective of this study was to analyze the behavior of silver nanoparticles stabilized with collagen (AgNPcol) and to check the skin permeation capacity and action in paw edema induced by carrageenan. AgNPcol synthesis was carried out using solutions of reducing agent sodium borohydride (NaBH4), silver nitrate (AgNO3) and collagen. Characterization was done by using dynamic light scattering (DLS) and X-ray diffraction (XRD) and AFM. Cellular viability testing was performed by using flow cytometry in human melanoma cancer (MV3) and murine fibroblast (L929) cells. The skin permeation study was conducted using a Franz diffusion cell, and the efficiency of AgNPcol against the formation of paw edema in mice was evaluated. The hydrodynamic diameter and zeta potential of AgNPcol were 140.7 +/- 7.8 nm and 20.1 +/- 0.7 mV, respectively. AgNPcol failed to induce early apoptosis, late apoptosis, and necrosis in L929 cells; however, it exhibited enhanced toxicity in cancer cells (MV3) compared to normal cells (1929). AgNPcol demonstrated increased toxicological effects in cancer MV3 cells, promoting skin permeation, and preventing paw edema. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 15/10267-0 - Caracterização estrutural de nanopartículas de Acetato de Carvacrila com aplicação esquistossomicida
Beneficiário:Larissa Fernandes Batista
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 14/02282-6 - Estudo da relação estrutura-atividade de alcalóides derivados da Pilocarpus microphyllus (Rutaceae) com ação contra Schistosoma mansoni
Beneficiário:Ana Carolina Mafud
Linha de fomento: Bolsas no Brasil - Pós-Doutorado