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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Polyamines regulate phosphorylation-dephosphorylation kinetics in a crustacean gill (Na+, K+)-ATPase

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Autor(es):
Lucena, Malson Neilson ; Garcon, Daniela Pereira ; Leite Fontes, Carlos Frederico ; McNamara, John Campbell ; Leone, Francisco Assis
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: Molecular and Cellular Biochemistry; v. 429, n. 1-2, p. 187-198, MAY 2017.
Citações Web of Science: 4
Resumo

Aiming to clarify the mechanism of inhibition of (Na+, K+)-ATPase activity by polyamines, we examined the effects of exogenous putrescine, spermidine, and spermine on the kinetic behavior of phosphoenzyme-linked partial reactions using a microsomal gill (Na+, K+)-ATPase from juvenile and adult M. amazonicum, a freshwater palaemonid shrimp. The time course of phosphointermediate formation is greater (0.089 +/- 0.006 s(-1)) in adults than in juveniles (0.053 +/- 0.003 s(-1)) for spermidine, but similar to juveniles (0.059 +/- 0.004 s(-1)) for putrescine. Maximum phosphointermediate formation for the (Na+, K+)-ATPase from juveniles decreased by 46% and 32% with spermidine and putrescine, respectively. In adults, maximum phosphointermediate levels decreased by 50% and 8%, respectively. For both spermidine and putrescine, dephosphorylation rates were higher for adults than for juveniles, and were higher than in controls without polyamines. Spermine had a negligible effect (<10%) on phosphorylation/dephosphorylation rates of both juvenile and adult enzymes. This is the first report on the effects of polyamines on phosphoenzyme-linked partial reactions in juvenile and adult M. amazonicum gill (Na+, K+)-ATPases. Our findings suggest that the phosphorylation/dephosphorylation steps of this gill enzyme may be regulated by polyamines during ontogenetic development. (AU)

Processo FAPESP: 13/24252-9 - O papel da (Na+,K+)-ATPase na capacidade osmorregulatória do caranguejo Ucides cordatus: uma abordagem bioquímica e molecular
Beneficiário:Malson Neilson de Lucena
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/17534-0 - Papel da (Na,K)-ATPase na osmorregulação e excreção de amônio em crustáceos eurialinos: identificação e efeitos do FXYD2 na regulação da (Na,K)-ATPase branquial do siri Callinectes danae
Beneficiário:Francisco de Assis Leone
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/06395-9 - Papel da (Na,K)-ATPase na osmorregulação e excreção de amônio em crustáceos eurialinos: identificação e efeitos do FXYD2 na regulação da (Na,K)-ATPase branquial do siri Callinectes danae
Beneficiário:Daniela Pereira Garçon
Linha de fomento: Bolsas no Brasil - Pós-Doutorado