Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Intrauterine Growth Restriction Programs the Hypothalamus of Adult Male Rats: Integrated Analysis of Proteomic and Metabolomic Data

Texto completo
Autor(es):
Mostrar menos -
Pedroso, Amanda P. ; Souza, Adriana P. ; Dornellas, Ana P. S. ; Oyama, Lila M. ; Nascimento, Claudia M. O. ; Santos, Gianni M. S. ; Rosa, Jose C. ; Bertolla, Ricardo P. ; Kla-Witter, Jelena ; Christians, Uwe ; Tashima, Alexandre K. ; Ribeire, Eliane B.
Número total de Autores: 12
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PROTEOME RESEARCH; v. 16, n. 4, p. 1515-1525, APR 2017.
Citações Web of Science: 8
Resumo

Programming of hypothalamic functions regulating energy homeostasis may play a role in intrauterine growth restriction (IUGR)-induced adulthood obesity. The present study investigated the effects of IUGR on the hypothalamus proteome and metabolome of adult rats submitted to 50% protein-energy restriction throughout pregnancy. Proteomic and metabolomic analyzes were performed by data independent acquisition mass spectrometry and multiple reaction monitoring, respectively. At age 4 months, the restricted rats showed elevated adiposity, increased leptin and signs of insulin resistance. 1356 proteins were identified and 348 quantified while 127 metabolites were quantified. The restricted hypothalamus showed down regulation of 36 proteins and 5 metabolites and up-regulation of 21 proteins and 9 metabolites: Integrated pathway analysis of the proteomics and metabolomics data indicated impairment of hypothalamic glucose metabolism, increased flux through the hexosamine pathway, deregulation of TCA cycle and the respiratory chain, and alterations in glutathione metabolism. The data suggest IUGR. modulation of energy metabolism and redox homeostasis in the hypothalamus of male adult rats: The present results indicated deleterious consequences of IUGR on hypothalamic pathways involved in pivotal physiological functions. These results provide guidance for future mechanistic studies assessing the role of intrauterine malnutrition in the development of metabolic diseases later in life. (AU)

Processo FAPESP: 12/19321-9 - Caracterização proteômica e peptidômica dos venenos de aranhas brasileiras por espectrometria de massas
Beneficiário:Alexandre Keiji Tashima
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/20268-0 - Expressão protéica no hipotálamo e tecido adiposo de ratos machos e fêmeas adultos expostos a desnutrição intrauterina
Beneficiário:Eliane Beraldi Ribeiro
Linha de fomento: Auxílio à Pesquisa - Regular