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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

NAD9/NAD7 (mitochondrial nicotinamide adenine dinucleotide dehydrogenase gene)-A new ``Holy Grail{''} phylogenetic and DNA-barcoding marker for Arcellinida (Amoebozoa)?

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Autor(es):
Blandenier, Quentin ; Lara, Enrique ; Mitchell, Edward A. D. ; Alcantara, Daniel M. C. ; Siemensma, Ferry J. ; Todorov, Milcho ; Lahr, Daniel J. G.
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF PROTISTOLOGY; v. 58, p. 175-186, APR 2017.
Citações Web of Science: 3
Resumo

Molecular phylogeny is an indispensable tool for assessing evolutionary relationships among protists. The most commonly used marker is the small subunit ribosomal RNA gene, a conserved gene present in many copies in the nuclear genomes. However, this marker is not variable enough at a fine-level taxonomic scale, and intra-genomic polymorphism has already been reported. Finding a marker that could be useful at both deep and fine taxonomic resolution levels seemed like a utopic dream. We designed Amoebozoa-specific primers to amplify a region including partial sequences of two subunits of the mitochondrial nicotinamide adenine dinucleotide dehydrogenase gene (NAD9/NAD7). We applied them to arcellinids belonging to distantly related genera (Arcella, Difflugia, Netzelia and Hyalosphenia) and to Arcellinid-rich environmental samples to obtain additional Amoebozoa sequences. Tree topology was congruent with previous phylogenies, all nodes being highly supported, suggesting that this marker is well-suited for deep phylogenies in Arcellinida and perhaps Amoebozoa. Furthermore, it enabled discrimination of close-related taxa. This short genetic marker (ca. 250 bp) can therefore be used at different taxonomic levels, due to a fast-varying intergenic region presenting either a small intergenic sequence or an overlap, depending on the species. (C) 2016 Elsevier GmbH. All rights reserved. (AU)

Processo FAPESP: 13/04585-3 - Decifrando as grandes tendências de evolução molecular e morfológica nos Amoebozoa
Beneficiário:Daniel José Galafasse Lahr
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores