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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Revisiting antithrombotic therapeutics; sculptin, a novel specific, competitive, reversible, scissile and tight binding inhibitor of thrombin

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Iqbal, Asif ; Goldfeder, Mauricio Barbugiani ; Marques-Porto, Rafael ; Asif, Huma ; de Souza, Jean Gabriel ; Faria, Fernanda ; Chudzinski-Tavassi, Ana Marisa
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 7, MAY 3 2017.
Citações Web of Science: 3

Thrombin is a multifunctional enzyme with a key role in the coagulation cascade. Its functional modulation can culminate into normal blood coagulation or thrombosis. Thus, the identification of novel potent inhibitors of thrombin are of immense importance. Sculptin is the first specific thrombin inhibitor identified in the transcriptomics analysis of tick's salivary glands. It consists of 168 residues having four similar repeats and evolutionary diverged from hirudin. Sculptin is a competitive, specific and reversible inhibitor of thrombin with a K-i of 18.3 +/- 1.9 pM (k(on) 4.04 +/- 0.03 x 10(7) M-1 s(-1) and k(off) 0.65 +/- 0.04 x 10(-3) s(-1)). It is slowly consumed by thrombin eventually losing its activity. Contrary, sculptin is hydrolyzed by factor Xa and each polypeptide fragment is able to inhibit thrombin independently. A single domain of sculptin alone retains similar to 45% of inhibitory activity, which could bind thrombin in a bivalent fashion. The formation of a small turn/helical-like structure by active site binding residues of sculptin might have made it a more potent thrombin inhibitor. In addition, sculptin prolongs global coagulation parameters. In conclusion, sculptin and its independent domain(s) have strong potential to become novel antithrombotic therapeutics. (AU)

Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
Beneficiário:Hugo Aguirre Armelin
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival
Beneficiário:Ana Marisa Chudzinski-Tavassi
Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia