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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Toxicological effects of graphene oxide on adult zebrafish (Danio rerio)

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Autor(es):
Souza, Jaqueline P. ; Baretta, Jessica F. ; Santos, Fabricio ; Paino, Ieda M. M. ; Zucolotto, Valtencir
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: AQUATIC TOXICOLOGY; v. 186, p. 11-18, MAY 2017.
Citações Web of Science: 17
Resumo

Graphene exhibits unique physical and chemical properties that facilitate its application in many fields, including electronics and biomedical areas. However, the use of graphene and its derivatives could result in accumulation in aquatic environments, and the risks posed by these compounds for organisms are not completely understood. In this study, we investigated the effects of graphene oxide (GO) on adult zebrafish (Danio rerio). Experimental fish were exposed to 2, 10 or 20 mg L-1 GO, and the cytotoxicity, genotoxicity and oxidative stress were assessed. The morphology of the gills and liver tissues was also analyzed. Graphene oxide exposure led to an increase in the number of gill cells that were in early apoptotic and necrotic stages, but genotoxicity was not observed in blood cells. We also observed the generation of Reactive Oxygen Species (ROS) in gill cells. Structural analysis revealed injuries to gill tissues, including a dilated marginal channel, lamellar fusion, clubbed tips, swollen mucocytes, epithelial lifting, aneurysms, and necrosis. Liver tissues also presented lesions such as peripherally located nuclei. Furthermore, hepatocytes exhibited a non-uniform shape, picnotic nuclei, vacuole formation, cell rupture, and necrosis. Our results showed that sub-lethal doses of graphene oxide could be harmful to fish species and thus represent risks for the aquatic food chain. (C) 2017 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 14/13493-8 - Avaliação dos efeitos tóxicos das nanopartículas óxido de grafeno e nanorods de ouro sobre organismos aquáticos
Beneficiário:Jaqueline Pérola de Souza
Linha de fomento: Bolsas no Brasil - Pós-Doutorado