Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Molecular characterization of short-term primary cultures and comparison with corresponding tumor tissue of Brazilian glioblastoma patients

Texto completo
Autor(es):
Mostrar menos -
Cruvinel-Carloni, Adriana ; Silva-Oliveira, Renato ; Torrieri, Raul ; Bidinotto, Lucas Tadeu ; Berardinelli, Gustavo Noriz ; Oliveira-Silva, Viviane Aline ; Clara, Carlos Afonso ; de Almeida, Gisele Caravina ; Martinho, Olga ; Squire, Jeremy Andrew ; Reis, Rui Manuel
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: TRANSLATIONAL CANCER RESEARCH; v. 6, n. 2, p. 332+, APR 2017.
Citações Web of Science: 4
Resumo

Background: Glioblastoma, the most frequent and malignant adult brain tumor, has been extensively studied. However, there is no effective treatment, and to overcome this challenging scenario, it is essential to improve preclinical biological models. This study aimed to molecularly characterize short-term glioblastoma primary cultures and to compare them with patient tumor profiles. Methods: Glioblastoma cell lines were established from Barretos Cancer Hospital patients diagnosed with glioblastoma. The cells were cultured with DMEM (+)10% FBS (+)1% PS and were molecularly characterized using array CGH (aCGH), next-generation and Sanger sequencing. Results: We established four short-term glioblastoma cultures and we found that the primary cells exhibited a diversity of chromosomal aberrations, with gain of chromosome 7 and loss of chromosomes 10, 13 and 17p being the most frequent alterations. Mutation profiling showed that hotspot TERT promoter mutations were present in 3/4 cases, followed by mutations in TP53 (2/4) and in the RB1, BRAF and PTEN (1/4) genes. A similar chromosomal and mutation pattern was observed in all short-term cultures and matched frozen tumors. Conclusions: Herein, short-term glioblastoma primary cultures were successfully characterized and had genetic make-ups that were similar to those of patient tumors, suggesting that short-term primary cultures are suitable in vitro models for studies of glioblastoma biology. (AU)

Processo FAPESP: 12/08287-4 - Perfil de alterações cromossômicas em gliomas
Beneficiário:Lucas Tadeu Bidinotto
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
Processo FAPESP: 11/08523-7 - Perfil de alterações cromossômicas em tumores cerebrais (meduloblastomas e gliomas): impacto na identificação de novas vias tumorigênicas
Beneficiário:Lucas Tadeu Bidinotto
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/19590-0 - Perfil mutacional de linhagens primárias de glioblastomas
Beneficiário:Rui Manuel Vieira Reis
Linha de fomento: Auxílio à Pesquisa - Regular