3,5-diiodothyronine (3,5-T2) reduces blood glucose independently of insulin sensitization in obese mice

Autor(es)

da Silva Teixeira, S. ; Filgueira, C. ; Sieglaff, D. H. ; Benod, C. ; Villagomez, R. ; Minze, L. J. ; Zhang, A. ; Webb, P. ; Nunes, M. T.

• Tipo de documento: Artigo Científico
• Fonte: ACTA PHYSIOLOGICA; v. 220, n. 2, p. 238-250, JUN 2017.
• Citações Web of Science: 18

Resumo

AimThyroid hormones regulate metabolic response. While triiodothyronine (T3) is usually considered to be the active form of thyroid hormone, one form of diiodothyronine (3,5-T2) exerts T3-like effects on energy consumption and lipid metabolism. 3,5-T2 also improves glucose tolerance in rats and 3,5-T2 levels correlate with fasting glucose in humans. Presently, however, little is known about mechanisms of 3,5-T2 effects on glucose metabolism. Here, we set out to compare effects of T3, 3,5-T2 and another form of T2 (3,3-T2) in a mouse model of diet-induced obesity and determined effects of T3 and 3,5-T2 on markers of classical insulin sensitization to understand how diiodothyronines influence blood glucose. MethodsCell- and protein-based assays of thyroid hormone action. Assays of metabolic parameters in mice. Analysis of transcript and protein levels in different tissues by qRT-PCR and Western blot. ResultsT3 and 3,5-T2 both reduce body weight, adiposity and body temperature despite increased food intake. 3,3-T2 lacks these effects. T3 and 3,5-T2 reduce blood glucose levels, whereas 3,3-T2 worsens glucose tolerance. Neither T3 nor 3,5-T2 affects markers of insulin sensitization in skeletal muscle or white adipose tissue (WAT), but both reduce hepatic GLUT2 glucose transporter levels and glucose output. T3 and 3,5-T2 also induce expression of mitochondrial uncoupling proteins (UCPs) 3 and 1 in skeletal muscle and WAT respectively. Conclusions3,5-T2 influences glucose metabolism in a manner that is distinct from insulin sensitization and involves reductions in hepatic glucose output and changes in energy utilization. (AU)

• Processo FAPESP: 10/18151-7 - Caracterização das ações dos HT e do seu agonista GC-1 na regulação da homeostase glicêmica: estudo em células musculares e em ratos diabéticos
• Beneficiário: Silvania da Silva Teixeira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/17430-5 - Caracterização das ações do T3 na regulação da homeostase glicídica
• Beneficiário: Silvania da Silva Teixeira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/05629-4 - Ações genômicas vs não genômicas dos hormônios tiroidianos: mudanças de paradigmas, implicações fisiológicas e perspectivas terapêuticas
• Beneficiário: Maria Tereza Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Temático