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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bacterial short-chain fatty acid metabolites modulate the inflammatory response against infectious bacteria

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Autor(es):
Correa, R. O. ; Vieira, A. ; Sernaglia, E. M. ; Lancellotti, M. ; Vieira, A. T. ; Avila-Campos, M. J. ; Rodrigues, H. G. ; Vinolo, M. A. R.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Cellular Microbiology; v. 19, n. 7 JUL 2017.
Citações Web of Science: 14
Resumo

Short-chain fatty acids (SCFAs), predominantly acetic, propionic, and butyric acids, are bacterial metabolites with an important role in the maintenance of homeostasis due to their metabolic and immunomodulatory actions. Some evidence suggests that they may also be relevant during infections. Therefore, we aimed to investigate the effects of SCFAs in the effector functions of neutrophils to an opportunistic pathogenic bacterium, Aggregatibacter actinomycetemcomitans. Using a subcutaneous model to generate a mono, isolated infection of A.actinomycetemcomitans, we demonstrated that the presence of the SCFAs in situ did not affect leukocyte accumulation but altered the effector mechanisms of migrating neutrophils by downregulating the production of cytokines, their phagocytic capacity, and killing the bacteria, thus impairing the containment of A.actinomycetemcomitans. Similar effects were observed with bacteria-stimulated neutrophils incubated with SCFAs in vitro. These effects were independent of free-fatty acid receptor 2 (FFAR2) activation, the main SCFA receptor expressed on neutrophils, occurring possibly through inhibition of histone deacetylases because similar effects were obtained by using histone deacetylase inhibitors, such as SAHA, MS-275, and RGFP 966. Considering the findings of this study, we hypothesized that in an infectious condition, SCFAs may exert a detrimental effect on the host by inhibiting neutrophil's effector functions. (AU)

Processo FAPESP: 12/10653-9 - Envolvimento dos ácidos graxos de cadeia curta e seu receptor (GPR43) na resposta imune a bactérias anaeróbias in vivo e in vitro
Beneficiário:Marco Aurélio Ramirez Vinolo
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores