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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mapping of estradiol binding sites through receptor micro-autoradiography in the endometrial stroma of early pregnant mice

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Autor(es):
Zorn, Telma M. T. ; Favaro, Rodolfo R. ; Soto-Suazo, Mauricio ; Stumpf, Walter E.
Número total de Autores: 4
Tipo de documento: Artigo Científico
Fonte: Histochemistry and Cell Biology; v. 148, n. 3, p. 257-272, SEP 2017.
Citações Web of Science: 0
Resumo

Estradiol triggers key biological responses in the endometrium, which rely on the presence and levels of its cognate receptors on target cells. Employing the receptor micro-autoradiography (RMAR) technique, we aimed to provide a temporal and spatial map of the functional binding sites for estradiol in the mouse endometrial stroma during early pregnancy. Uterine samples from days 1.5 to 7.5 of pregnancy were collected 1 h after tritiated-(3H-) estradiol administration and prepared for RMAR analysis. Autoradiographic incorporation of 3H-thymidine (after 1-h pulse) was evaluated over the same gestational interval. Combined RMAR with either histochemistry with Dolichus biflorus (DBA) lectin or immunohistochemistry for detection of the desmin further characterized 3H-estradiol binding pattern in uterine Natural Killer (uNK) and decidual cells, respectively. 3H-estradiol binding levels oscillated in the pregnant endometrial stroma between the mesometrial and antimesometrial regions as well as the superficial and deep domains. Although most of the endometrial stromal cells retained the hormone, a sub-population of them, as well as endothelial and uNK cells, were unable to do so. Rises in the levels of 3H-estradiol binding preceded endometrial stromal cell proliferation. 3H-estradiol binding and 3H-thymidine incorporation progressively decreased along the development of the antimesometrial decidua. Endothelial proliferation occurred regardless of 3H-estradiol binding, whereas pericytes proliferation was associated with high levels of hormone binding. Endometrial cell populations autonomously control their levels of 3H-estradiol binding and retention, a process associated with their proliferative competence. Collectively, our results illustrate the intricate regulatory dynamic of nuclear estrogen receptors in the pregnant mouse endometrium. (AU)

Processo FAPESP: 11/22429-3 - Avaliação do impacto do diabetes tipo 1 sobre a resposividade dos tecidos uterinos à estimulação por estrógeno
Beneficiário:Rodolfo Favaro Ribeiro
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 99/00098-1 - Receptors for ovarian hormones in endometrial cells of mice.
Beneficiário:Telma Maria Tenório Zorn
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 99/00097-5 - Walter Stumpf | University North Carolina Chapel Hill/USA - Estados Unidos
Beneficiário:Telma Maria Tenório Zorn
Linha de fomento: Auxílio à Pesquisa - Pesquisador Visitante - Internacional