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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bergenin from Peltophorum dubium: Isolation, Characterization, and Anti-oxidant Activities in Non-Biological Systems and Erythrocytes

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de Oliveira, Guilherme A. L. ; da Silva Oliveira, George L. ; Nicolau, Lucas A. D. ; Mafud, Ana C. ; Batista, Larissa F. ; Mascarenhas, Yvonne P. ; de Souza, Luan K. M. ; David, Jorge M. ; Pinto, Larissa S. ; Alves, Clayton Q. ; Medeiros, Jand V. R.
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Medicinal Chemistry; v. 13, n. 6, p. 592-603, 2017.
Citações Web of Science: 1
Resumo

Background: Bergenin, a compound derived from gallic acid, is a secondary metabolite of the plant Peltophorum dubium (Spreng.) Taub. Objective: In this study, we aimed to characterize the ability of bergenin to eliminate the radicals in non-biological systems. Methods: We evaluated bergenin's ability to protect erythrocytes from oxidative damage in a biological system. We have elucidated bergenin structure using nuclear magnetic resonance, X-ray diffraction, Fourier transform infrared spectroscopy, and differential scanning calorimetry. We then evaluated its antioxidant capacity in vitro against DPPH center dot, ABTS(center dot+), hydroxyl radicals, and nitric oxide, and determined its ability to transfer electrons owing to its reduction potential and ability to chelate iron. We also evaluated its protective capacity against oxidative damage produced by AAPH in erythrocytes, its hemolytic properties, its ability to inhibit hemolysis, and its ability to maintain intracellular reduced glutathione homeostasis. Results: Bergenin concentrations between 0.1 and 3mM significantly (p < 0.05) and dose dependently decreased formation of ABTS(center dot+), DPPH center dot, nitrite ions, OH center dot, reduced formation ferricyanide, ferrozine-Fe2+ complex, inhibited AAPH-induced oxidative hemolysis of erythrocytes, raised GSH levels in the presence of AAPH, inhibited AAPH-induced lipid peroxidation in erythrocytes. Conclusion: Bergenin may represent a novel alternative antioxidant, with potential applications in various industries, including drugs, cosmetics, and foods. (AU)

Processo FAPESP: 14/02282-6 - Estudo da relação estrutura-atividade de alcalóides derivados da Pilocarpus microphyllus (Rutaceae) com ação contra Schistosoma mansoni
Beneficiário:Ana Carolina Mafud
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/18023-5 - Estudos pré-clínicos da Epiisopiloturina e epiisopilosina e seus efeitos contra outros helmintos
Beneficiário:Ana Carolina Mafud
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado