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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata

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Autor(es):
Milani, Diogo ; Ramos, Erica ; Loreto, Vilma ; Andrea Marti, Dardo ; Cardoso, Adauto Lima ; Martinez de Moraes, Karen Cristiane ; Martins, Cesar ; Cabral-de-Mello, Diogo Cavalcanti
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: BMC GENETICS; v. 18, AUG 29 2017.
Citações Web of Science: 1
Resumo

Background: Satellite DNAs (satDNAs) are organized in repetitions directly contiguous to one another, forming long arrays and composing a large portion of eukaryote genomes. These sequences evolve according to the concerted evolution model, and homogenization of repeats is observed at the intragenomic level. Satellite DNAs are the primary component of heterochromatin, located primarily in centromeres and telomeres. Moreover, satDNA enrichment in specific chromosomes has been observed, such as in B chromosomes, that can provide clues about composition, origin and evolution of this chromosome. In this study, we isolated and characterized a satDNA in A and B chromosomes of Abracris flavolineata by integrating cytogenetic, molecular and genomics approaches at intra-and inter-population levels, with the aim to understand the evolution of satDNA and composition of B chromosomes. Results: AflaSAT-1 satDNA was shared with other species and in A. flavolineata, was associated with another satDNA, AflaSAT-2. Chromosomal mapping revealed centromeric blocks variable in size in almost all chromosomes (except pair 11) of A complement for both satDNAs, whereas for B chromosome, only a small centromeric signal occurred. In distinct populations, variable number of AflaSAT-1 chromosomal sites correlated with variability in copy number. Instead of such variability, low sequence diversity was observed in A complement, but monomers from B chromosome were more variable, presenting also exclusive mutations. AflaSAT-1 was transcribed in five tissues of adults in distinct life cycle phases. Conclusions: The sharing of AflaSAT-1 with other species is consistent with the library hypothesis and indicates common origin in a common ancestor; however, AflaSAT-1 was highly amplified in the genome of A. flavolineata. At the population level, homogenization of repeats in distinct populations was documented, but dynamic expansion or elimination of repeats was also observed. Concerning the B chromosome, our data provided new information on the composition in A. flavolineata. Together with previous results, the sequences of heterochromatic nature were not likely highly amplified in the entire B chromosome. Finally, the constitutive transcriptional activity suggests a possible unknown functional role, which should be further investigated. (AU)

Processo FAPESP: 15/16661-1 - Cromossomos sexuais, cromossomos B e seus enigmas: sistemas modelo para estudos de evolução cromossômica e genômica
Beneficiário:César Martins
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/05246-3 - Organização de DNAs satélites no genoma do gafanhoto Abracris flavolineata com ênfase nos cromossomos supranumerários: uma abordagem estrutural, funcional e evolutiva
Beneficiário:Diogo Milani
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 11/19481-3 - Contribuições a elucidação do significado biológico de cromossomos B utilizando como modelos Rhammatocerus brasiliensis (Orthoptera) e Haplochromis obliquidens (Teleostei): estrutura, evolução e padrões de modificações em proteínas histônicas
Beneficiário:Diogo Cavalcanti Cabral de Mello
Linha de fomento: Auxílio à Pesquisa - Regular